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Dynamic peptide-fol...
Dynamic peptide-folding mediated biofunctionalization and modulation of hydrogels for 4D bioprinting
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- Aronsson, Christopher (author)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
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- Jury, Michael (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Naeimipour, Sajjad (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Rasti Boroojeni, Fatemeh (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Christoffersson, Jonas, 1986- (author)
- Linköpings universitet,Högskolan i Skövde,Institutionen för biovetenskap,Forskningsmiljön Systembiologi,Division of Biotechnology, Department of Physics, Chemistry and Biology, Linköping University, Sweden,Translationell Bioinformatik, Translational Bioinformatics,Teknisk biologi,Tekniska fakulteten,Univ Skovde, Sweden
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- Lifwergren, Philip (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Mandenius, Carl-Fredrik (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Selegård, Robert (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Aili, Daniel (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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(creator_code:org_t)
- 2020-06-29
- 2020
- English.
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In: Biofabrication. - : Institute of Physics Publishing (IOPP). - 1758-5082 .- 1758-5090. ; 12:3
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Abstract
Subject headings
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- Hydrogels are used in a wide range of biomedical applications, including three-dimensional (3D) cell culture, cell therapy and bioprinting. To enable processing using advanced additive fabrication techniques and to mimic the dynamic nature of the extracellular matrix (ECM), the properties of the hydrogels must be possible to tailor and change over time with high precision. The design of hydrogels that are both structurally and functionally dynamic, while providing necessary mechanical support is challenging using conventional synthesis techniques. Here, we show a modular and 3D printable hydrogel system that combines a robust but tunable covalent bioorthogonal cross-linking strategy with specific peptide-folding mediated interactions for dynamic modulation of cross-linking and functionalization. The hyaluronan-based hydrogels were covalently cross-linked by strain-promoted alkyne-azide cycloaddition using multi-arm poly(ethylene glycol). In addition, a de novo designed helix-loop-helix peptide was conjugated to the hyaluronan backbone to enable specific peptide-folding modulation of cross-linking density and kinetics, and hydrogel functionality. An array of complementary peptides with different functionalities was developed and used as a toolbox for supramolecular tuning of cell-hydrogel interactions and for controlling enzyme-mediated biomineralization processes. The modular peptide system enabled dynamic modifications of the properties of 3D printed structures, demonstrating a novel route for design of more sophisticated bioinks for four-dimensional bioprinting. © 2020 The Author(s). Published by IOP Publishing Ltd.
Subject headings
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biomaterial (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bio Materials (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- biomineralization
- bioprinting
- folding
- hydrogel
- hyaluronan
- peptide
- Cell culture
- Hyaluronic acid
- Hydrogels
- Medical applications
- Modulation
- Peptides
- Polyethylene glycols
- Biomedical applications
- Biomineralization process
- Conventional synthesis
- Covalently cross-linked
- Cross-linking density
- Dynamic modifications
- Extracellular matrices
- Three-dimensional (3d) cell culture
- 3D printers
- Bioinformatik
- Bioinformatics
Publication and Content Type
- ref (subject category)
- art (subject category)
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