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Diphenyleneiodonium...
Diphenyleneiodonium efficiently inhibits the characteristics of a cancer stem cell model derived from induced pluripotent stem cells
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- Monzur, Sadia (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan
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- Hassan, Ghmkin (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan
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- Afify, Said M. (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan ; Chemistry Department, Division of Biochemistry, Faculty of Science, Menoufia University, Shebin El Kom-Menoufia, Egypt
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- Kumon, Kazuki (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan
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- Mansour, Hager (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan
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- Nawara, Hend M. (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan
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- Sheta, Mona (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan ; Department of Cancer Biology, National Cancer Institute, Cairo University, Egypt
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- Abu Quora, Hagar A. (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan ; Cytology, Histology and Histochemistry, Zoology Department, Faculty of Science, Menoufia University, Shebin El Kom-Menoufia, Egypt
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- Zahra, Maram H. (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan
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- Xu, Yanning (author)
- Tianjin Key Laboratory of Human Development and Reproductive Regulation, Department of Pathology, Tianjin Central Hospital of Gynecology Obstetrics, Nankai University Affiliated Maternity Hospital, Tianjin, China
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- Fu, Xiaoyin (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan ; Department of Pathology, Tianjin University of Traditional Chinese Medicine, Tianjin, China
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- Seno, Akimasa (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan ; R&D Division, The Laboratory of Natural Food & Medicine Co., Ltd, Okayama University Incubator, Japan
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- Wikström, Per (author)
- Glucox Biotech AB, Färentuna, Sweden
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- Szekeres, Ferenc L. M. (author)
- Högskolan i Skövde,Institutionen för hälsovetenskaper,Forskningsmiljön hälsa, hållbarhet och digitalisering,Translationell medicin TRIM, Translational Medicine
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- Seno, Masaharu (author)
- Department of Biotechnology and Drug Discovery, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Japan
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(creator_code:org_t)
- 2022-03-14
- 2022
- English.
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In: Cell Biochemistry and Function. - : John Wiley & Sons. - 0263-6484 .- 1099-0844. ; 40:3, s. 310-320
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Diphenyleneiodonium (DPI) has long been evaluated as an anticancer drug inhibiting NADPH oxidase, the IC50 in several cancer cell lines was reported 10 µM, which is too high for efficacy. In this study, we employed miPS-Huh7cmP cells, which we previously established as a cancer stem cell (CSC) model from induced pluripotent stem cells, to reevaluate the efficacy of DPI because CSCs are currently one of the main foci of therapeutic strategy to treat cancer, but generally considered resistant to chemotherapy. As a result, the conventional assay for the cell growth inhibition by DPI accounted for an IC50 at 712 nM that was not enough to define the effectiveness as an anticancer drug. Simultaneously, the wound-healing assay revealed an IC50 of approximately 500 nM. Comparatively, the IC50 values shown on sphere formation, colony formation, and tube formation assays were 5.52, 12, and 8.7 nM, respectively. However, these inhibitory effects were not observed by VAS2780, also a reputed NADPH oxidase inhibitor. It is noteworthy that these three assays are evaluating the characteristic of CSCs and are designed in the three-dimensional (3D) culture methods. We concluded that DPI could be a suitable candidate to target mitochondrial respiration in CSCs. We propose that the 3D culture assays are more efficient to screen anti-CSC drug candidates and better mimic tumor microenvironment when compared to the adherent monolayer of 2D culture system used for a conventional assay, such as cell growth inhibition and wound-healing assays. © 2022 John Wiley & Sons Ltd.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- 2D culture
- 3D culture
- cancer stem cell
- colony formation
- differentiation
- diphenyleneiodonium chloride
- sphere formation
- Translationell medicin TRIM
- Translational Medicine TRIM
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Monzur, Sadia
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Hassan, Ghmkin
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Afify, Said M.
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Kumon, Kazuki
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Mansour, Hager
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Nawara, Hend M.
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show more...
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Sheta, Mona
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Abu Quora, Hagar ...
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Zahra, Maram H.
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Xu, Yanning
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Fu, Xiaoyin
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Seno, Akimasa
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Wikström, Per
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Szekeres, Ferenc ...
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Seno, Masaharu
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University of Skövde