Rapid identification of pathogenic yeast isolates bt real-time PCR and two-dimensional melting-point analysis

Bergman, A.Department of Clinical Microbiology, Capio Diagnostik AB, Kärnsjukhuset, Skövde (author)

There is a need in the clinical microbiological laboratory for rapid and reliable methods for the universal identification of fungal pathogens. Two different regions of the rDNA gene complex, the highly polymorphic ITS1 and ITS2, were amplified using primers targeting conserved regions of the 18S, 5.8S and 28S genes. After melting-point analysis of the amplified products, the Tm of the two PCR-products were plotted into a spot diagram where all the 14 tested, clinically relevant yeasts separated with good resolution. Real-time amplification of two separate genes, melting-point analysis and two-dimensional plotting of Tm data can be used as a broad-range method for the identification of clinical isolates of pathogenic yeast such as Candida and Cryptococcus spp.

Fernandez, V.Department of Parasitology, Mycology and Environmental Microbiology, Swedish Institute for Infectious Disease Control, Solna (author)
Holmström, Kjell-OveHögskolan i Skövde,Forskningscentrum för Systembiologi,Institutionen för vård och natur(Swepub:his)holl (author)
Claesson, B. E. B.Department of Clinical Microbiology, Capio Diagnostik AB, Kärnsjukhuset, Skövde (author)
Enroth, H.Department of Clinical Microbiology, Capio Diagnostik AB, Kärnsjukhuset, Skövde (author)
Department of Clinical Microbiology, Capio Diagnostik AB, Kärnsjukhuset, SkövdeDepartment of Parasitology, Mycology and Environmental Microbiology, Swedish Institute for Infectious Disease Control, Solna (creator_code:org_t)

In:European Journal of Clinical Microbiology and Infectious Diseases: Springer26:11, s. 813-8180934-97231435-4373

By the author/editor: Bergman, A.; Fernandez, V.; Holmström, Kjell ...; Claesson, B. E. ...; Enroth, H.

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