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Aberrant promoter m...
Aberrant promoter methylation and loss of suppressor of cytokine signalling-1 gene expression in the development of uterine cervical carcinogenesis
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- Sobti, R. C. (author)
- Department of Biotechnology, Panjab University, Sector-14, Chandigarh, India
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- Singh, Neha (author)
- Department of Biotechnology, Panjab University, Sector-14, Chandigarh, India
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- Hussain, Showket (author)
- Division of Molecular Genetics and Biochemistry, Institute of Cytology & Preventive Oncology (ICMR), I-7, Sector-39, Noida, India
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- Suri, Vanita (author)
- Department of Gynaecology and Obstetrics, PGIMER, Sector-12, Chandigarh, India
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- Nijhawan, Raje (author)
- Department of Cytology & Gynae Pathology, PGIMER, Sector-12, Chandigarh, India
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- Bharti, A. C. (author)
- Division of Molecular Oncology, Institute of Cytology & Preventive Oncology (ICMR), I-7, Sector-39, Noida, India
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- Bharadwaj, Mausumi (author)
- Division of Molecular Genetics and Biochemistry, Institute of Cytology & Preventive Oncology (ICMR), I-7, Sector-39, Noida, India
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- Das, B. C. (author)
- Dr. B.R. Ambedkar Research Centre for Biomedical Research (ACBR), University of Delhi, (North Campus), Delhi, India
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(creator_code:org_t)
- 2011-09-21
- 2011
- English.
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In: Cellular Oncology. - : Springer Science and Business Media LLC. - 2211-3428 .- 2211-3436. ; 34:6, s. 533-43
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- BACKGROUND: Cervical cancer is a leading cause of cancer related deaths in women worldwide caused due to infection of high-risk human papillomaviruses. As JAK/STAT signalling pathway has been shown to play an important role during carcinogenesis, we studied the role of silencing of Suppressor of Cytokine Signalling-1 (SOCS-1) gene, a negative regulator of JAK/STAT pathway in cervical cancer.METHODS: The expression pattern of SOCS-1 mRNA and protein was analyzed in different stages of cervical tumor biopsies while normal cervical tissues served as controls. RT-PCR, immunohistochemistry and methylation-specific PCR (MSP) were performed to assess the expression pattern and promoter methylation of SOCS-1 gene in a total of 120 fresh surgically resected cervical tissue specimens comprising precancer (n = 12), cancer (n = 78) and normal controls (n = 30).RESULTS: Compared with expression of SOCS-1 in normal tissues, 64% of the tumor tissues expressed either undetectable or reduced expression. Aberrant promoter methylation of SOCS-1 was found in 61% of the cervical tumor tissues. SOCS-1 expression and methylation were significantly associated with severity of the disease (p < 0.01).CONCLUSION: We demonstrate for the first time the transcriptional inactivation of SOCS-1 gene due to hypermethylation and synergism with HPV infection which may play an important role in cervical carcinoma.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Cervical cancer
- SOCS-1
- HPV
- Promoter methylation
Publication and Content Type
- ref (subject category)
- art (subject category)
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