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Quantitative Proteomics of Yeast Post-Golgi Vesicles Reveals a Discriminating Role for Sro7p in Protein Secretion
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- Forsmark, Annabelle, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology, Microbiology, University of Gothenburg, Gothenburg, Sweden
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- Rossi, Guendalina (author)
- Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC, USA
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- Wadskog, Ingrid (author)
- Jönköping University,JTH, Kemiteknik
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- (creator_code:org_t)
- 2011-04-08
- 2011
- English.
- In: Traffic. - : John Wiley & Sons. - 1398-9219 .- 1600-0854. ; 12:6, s. 740-753
- Journal article (peer-reviewed)
Abstract
Subject headings
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- We here report the first comparative proteomics of purified yeast post-Golgi vesicles (PGVs). Vesicle samples isolated from PGV-accumulating sec6-4 mutants were treated with isobaric tags (iTRAQ) for subsequent quantitative tandem mass spectrometric analysis of protein content. After background subtraction, a total of 66 vesicle-associated proteins were identified, including known or assumed vesicle residents as well as a fraction not previously known to be PGV associated. Vesicles isolated from cells lacking the polarity protein Sro7p contained essentially the same catalogue of proteins but showed a reduced content of a subset of cargo proteins, in agreement with a previously shown selective role for Sro7p in cargo sorting.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Exocytosis
- Golgi
- ITRAQ
- Membrane trafficking
- Proteomics
- Vesicles
- cell protein
- protein sro7p
- unclassified drug
- article
- cell vacuole
- Golgi complex
- nonhuman
- priority journal
- protein analysis
- protein content
- protein secretion
- protein targeting
- quantitative analysis
- tandem mass spectrometry
- yeast
- Adaptor Proteins
- Signal Transducing
- Biological Markers
- Cytoplasmic Vesicles
- Golgi Apparatus
- Protein Transport
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Adaptor Proteins
- Signal Transducing
- genetics
- metabolism
- Biological Markers
- Cytoplasmic Vesicles
- chemistry
- Golgi Apparatus
- Protein Transport
- Proteomics
- methods
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- cytology
- Saccharomyces cerevisiae Proteins
- classification
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- art (subject category)
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