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Lab-on-DVD :
Lab-on-DVD : standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics
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- Ramachandraiah, Harisha (author)
- KTH,Proteomik,Science for Life Laboratory, SciLifeLab
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- Amasia, Mary (author)
- KTH,Science for Life Laboratory, SciLifeLab
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Cole, Jackie (author)
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Sheard, Paul (author)
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Pickhaver, Simon (author)
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Walker, Chris (author)
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Wirta, Valtteri (author)
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Lexow, Preben (author)
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Lione, Richard (author)
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- Russom, Aman (author)
- KTH,Proteomik,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- Royal Society of Chemistry (RSC), 2013
- 2013
- English.
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In: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 13:8, s. 1578-1585
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- We present a novel "Lab-on-DVD" system and demonstrate its capability for rapid and low-cost HIV diagnostics by counting CD4+ cells isolated from whole blood. We show that a commercial DVD drive can, with certain modifications, be turned into an improved DVD-based laser scanning microscope (DVD-LSM). The system consists of a multi-layered disposable polymer disc and a modified commercial DVD reader with rotational control for sample handling, temperature control for optimized bioassay, a photodiode array for detection, and software for signal processing and user interface - all the necessary components required for a truly integrated lab-on-a-chip system, with the capability to deliver high-resolution images down to 1 mm in size. Using discs modified with antibodies, we specifically captured CD4+ cells from whole blood, demonstrating single cell resolution imaging. The novel integrated DVD platform with sub-micron image resolution brings, for the first time, affordable cellular diagnostic testing to the point-of-care and should be readily applicable at resource-limited settings.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Platform
- Microchip
- Device
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Ramachandraiah, ...
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Amasia, Mary
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Cole, Jackie
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Sheard, Paul
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Pickhaver, Simon
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Walker, Chris
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show more...
-
Wirta, Valtteri
-
Lexow, Preben
-
Lione, Richard
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Russom, Aman
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Lab on a Chip
- By the university
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Royal Institute of Technology