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Characterization of...
Characterization of new fluorescent labels for ultra-high resolution microscopy
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- Chmyrov, Andriy, 1979- (author)
- KTH,Experimentell biomolekylär fysik
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- Arden-Jacob, Jutta (author)
- ATTO-TEC GmbH, Germany
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- Zilles, Alexander (author)
- University of Siegen, Germany
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- Drexhage, Karl-Heinz (author)
- University of Siegen, Germany
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- Widengren, Jerker (author)
- KTH,Experimentell biomolekylär fysik
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(creator_code:org_t)
- 2008-11-01
- 2008
- English.
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In: Photochemical and Photobiological Sciences. - : Springer Science and Business Media LLC. - 1474-905X .- 1474-9092. ; 7:11, s. 1378-1385
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Photo-induced switching of dyes into dark, long-lived states, such as a triplet state, has recently gained increasing interest, as a means to achieve ultra-high optical resolution. Additionally, these long lived states are often highly environment-sensitive and their photodynamics can thus offer additional independent fluorescence-based information. However, although providing a useful mechanism for photo-induced switching, the triplet state often appears as a precursor state for photobleaching, which potentially can limit its usefulness. In this work, a set of rhodamine and pyronin dyes, modified by substitution of heavy atoms and nitrogen within or close to the central xanthene unit of the dyes, were investigated with respect to their triplet state dynamics and photostabilities, under conditions relevant for ultra-high resolution microscopy. Out of the dyes investigated, in particular the rhodamine and pyronin dyes with a sulfur atom replacing the central oxygen atom in the xanthene unit were found to meet the requirements for ultra-high resolution microscopy, combining a prominent triplet state yield with reasonable photostability.
Subject headings
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Keyword
- fluorescence correlation spectroscopy
- super resolution
- tripet state
- GSD
- Spectroscopy
- Spektroskopi
Publication and Content Type
- ref (subject category)
- art (subject category)
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