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Visual DNA as a dia...
Visual DNA as a diagnostic tool
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- Pettersson, Erik (author)
- KTH,Genteknologi
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- Ståhl, Patrik L. (author)
- KTH,Genteknologi
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- Mahdessian, Hovsep (author)
- KTH,Genteknologi
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- Käller, Max (author)
- KTH,Genteknologi
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- Lundeberg, Joakim (author)
- KTH,Genteknologi
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- Ahmadian, Afshin (author)
- KTH,Genteknologi
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(creator_code:org_t)
- Wiley, 2009
- 2009
- English.
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In: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 30:21, s. 3691-3695
- Related links:
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http://www3.intersci...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- We report on the incorporation of the Visual DNA concept in a genotyping assay as a simple and straightforward detection tool. The principle of trapping streptavidin-coated superparamagnetic beads of micrometer size for visualization of genetic variances is used for PrASE-based detection of a panel of mutations in the severe and common genetic disorder of cystic fibrosis. The method allows a final investigation of genotypes by the naked eye and the output is easily documented using a regular hand-held device with an integrated digital camera. A number of samples were run through the assay, showing rapid and accurate detection using superparamagnetic beads and an off-the-shelf neodymium magnet. The assay emphasizes the power of Visual DNA and demonstrates the potential value of the method in future point-of-care tests.
Keyword
- Cystic fibrosis
- Diagnostics
- Mutation
- PrAse
- Visual DNA
- CYSTIC-FIBROSIS GENE
- IDENTIFICATION
- TECHNOLOGIES
Publication and Content Type
- ref (subject category)
- art (subject category)
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