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Parallel Fluorescence Correlation Spectroscopy with a fully integratedsingle photon 2x2 detector array made with conventional CMOS technology
- Serov, Alexandre (author)
- Gösch, Michael (author)
- Rochas, Alexis (author)
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- Anhut, Tiemo (author)
- Besse, Pierre-André (author)
- Popovi, Radivoje S. (author)
- Lasser, Theo (author)
- Rigler, Rudolf (author)
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- (creator_code:org_t)
- SPIE, 2003
- 2003
- English.
- In: Optical Technologies in Biophysics and Medicine V. - : SPIE. - 0819453978 ; , s. 248-258
- Conference paper (peer-reviewed)
Abstract
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- We present multipoint Fluorescence Correlation Spectroscopy (FCS) experiments with a fully integrated Complementary Metal Oxide Semiconductor (CMOS) single photon 2x2 detector array. Multifocal excitation was achieved with a diffractive optical element (DOE). Special emphasis was put on parallelization of the total system. In particular the performance of the single-photon CMOS detector was investigated and compared to a state-of-the art single-photon detecting module (actively quenched avalanche photo diode) by measurements on free difftising molecules at different concentrations. The potential of our new technique for high throughput FCS based systems is discussed.
Keyword
- Fluorescent Correlation Spectroscopy
- single-molecule detection
- single-photon detector
- CMOS technology
- diffractive optics
- TECHNOLOGY
- TEKNIKVETENSKAP
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