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Active liquid degas...
Active liquid degassing in microfluidic systems
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- Karlsson, J. Mikael, 1982- (author)
- KTH,Mikro- och nanosystemteknik,Microsystem Technology Lab
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- Gazin, Muriel (author)
- Universiteit Antwerpen,Laboratory of Medical Microbiology
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- Laakso, Sanna (author)
- Mobidiag Ltd
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- Haraldsson, Tommy (author)
- KTH,Mikro- och nanosystemteknik
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- Malhotra-Kumar, Surbhi (author)
- Universiteit Antwerpen,Laboratory of Medical Microbiology
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- Mäki, Minna (author)
- Orion Diagnostica Oy
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- Goossens, Herman (author)
- Universiteit Antwerpen,Laboratory of Medical Microbiology
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- van der Wijngaart, Wouter (author)
- KTH,Mikro- och nanosystemteknik
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(creator_code:org_t)
- RSC Publishing, 2013
- 2013
- English.
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In: Lab on a Chip. - : RSC Publishing. - 1473-0197 .- 1473-0189. ; 13:22, s. 4366-4373
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http://pubs.rsc.org/...
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https://kth.diva-por... (primary) (Raw object)
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http://kth.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- We present a method for efficient air bubble removal in microfluidic applications. Air bubbles are extracted from a liquid chamber into a vacuum chamber through a semipermeable membrane, consisting of PDMS coated with amorphous Teflon (R) AF 1600. Whereas air is efficiently extracted through the membrane, water loss is greatly reduced by the Teflon even at elevated temperatures. We present the water loss and permeability change with the amount of added Teflon AF to the membrane. Also, we demonstrate bubble-free, multiplex DNA amplification using PCR in a PDMS microfluidic device.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Air Bubble Formation
- Pcr
- Devices
- Chip
- Channels
- Removal
- Valves
- Trap
- Flow
Publication and Content Type
- ref (subject category)
- art (subject category)
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