Spatial organization of proteins in metastasizing cells

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Fältnamn	Indikatorer	Metadata
000		04300naa a2200565 4500
001		oai:DiVA.org:kth-129103
003		SwePub
008		130919s2013 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:prod.swepub.kib.ki.se:127273516
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-1291032 URI
024	7	a https://doi.org/10.1002/cyto.a.223042 DOI
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:1272735162 URI
040		a (SwePub)kthd (SwePub)ki
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Rönnlund, Danielu KTH,Experimentell biomolekylär fysik4 aut0 (Swepub:kth)u1vmbh2t
245	1 0	a Spatial organization of proteins in metastasizing cells
264		c 2013-05-08
264	1	b John Wiley & Sons,c 2013
338		a electronic2 rdacarrier
500		a QC 20130920
520		a The ability of tumor cells to invade into the surrounding tissue is linked to defective adhesive and mechanical properties of the cells, which are regulated by cell surface adhesions and the intracellular filamentous cytoskeleton, respectively. With the aim to further reveal the underlying mechanisms and provide new strategies for early cancer diagnostics, we have used ultrahigh resolution stimulated emission depletion (STED) microscopy as a means to identify metastasizing cells, based on their subcellular protein distribution patterns reflecting their specific adhesive and mechanical properties. We have compared the spatial distribution of cell-matrix adhesion sites and the vimentin filamentous systems in a matched pair of primary, normal, and metastatic human fibroblast cells. We found that the metastatic cells showed significantly increased densities and more homogenous distributions of nanoscale adhesion-related particles. Moreover, they showed an increase in the number but reduced sizes of the areas of cell-matrix adhesion complexes. The organization of the vimentin intermediate filaments was also found to be significantly different in the metastasizing cells, showing an increased entanglement and loss of directionality. Image analysis procedures were established, allowing an objective detection and characterization of these features and distinction of metastatic cells from their normal counterparts. In conclusion, our results suggest that STED microscopy provides a novel tool to identify metastasizing cells from a very sparse number of cells, based on the altered spatial distribution of the cell-matrix adhesions and intermediate filaments.
650	7	a NATURVETENSKAPx Biologix Cellbiologi0 (SwePub)106042 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Cell Biology0 (SwePub)106042 hsv//eng
650	7	a NATURVETENSKAPx Biologix Biofysik0 (SwePub)106032 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Biophysics0 (SwePub)106032 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng
653		a STED microscopy
653		a cell adhesion
653		a metastasis
653		a cancer
653		a diagnostics
653		a vimentin
653		a image analysis
653		a Biological Physics
653		a Biologisk fysik
700	1	a Gad, Annica K. B.u Karolinska Institutet4 aut
700	1	a Blom, Hansu KTH,Experimentell biomolekylär fysik4 aut0 (Swepub:kth)u1qhk3ta
700	1	a Aspenström, Pontusu Karolinska Institutet4 aut
700	1	a Widengren, Jerker,d 1965-u KTH,Experimentell biomolekylär fysik4 aut0 (Swepub:kth)u1i3g09c
710	2	a KTHb Experimentell biomolekylär fysik4 org
773	0	t Cytometry Part Ad : John Wiley & Sonsg 83:9, s. 855-865q 83:9<855-865x 1552-4922x 1552-4930
856	4	u https://kth.diva-portal.org/smash/get/diva2:650299/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint
856	4	u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cyto.a.22304
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-129103
856	4 8	u https://doi.org/10.1002/cyto.a.22304
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:127273516

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By the author/editor: Rönnlund, Daniel; Gad, Annica K. B ...; Blom, Hans; Aspenström, Pont ...; Widengren, Jerke ...

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Cell Biology

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cancer and Oncol ...

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By the university: Royal Institute of Technology; Karolinska Institutet

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