Search: onr:"swepub:oai:DiVA.org:kth-136077" >
Lognormality and os...
Lognormality and oscillations in the coverage of high-throughput transcriptomic data towards gene ends
-
- Innocenti, Nicolas, 1986- (author)
- KTH,Beräkningsbiologi, CB,Computational Biological Physics, CBP
-
- Aurell, Erik, 1961- (author)
- KTH,Beräkningsbiologi, CB,Computational Biological Physics, CBP
-
(creator_code:org_t)
- Institute of Physics (IOP), 2013
- 2013
- English.
-
In: Journal of Statistical Mechanics. - : Institute of Physics (IOP). - 1742-5468. ; 2013:10, s. P10013-
- Related links:
-
http://iopscience.io...
-
show more...
-
http://arxiv.org/abs...
-
https://kth.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- High-throughput transcriptomics experiments have reached the stage where the count of the number of reads alignable to a given position can be treated as an almost-continuous signal. This allows us to ask questions of biophysical/biotechnical nature, but which may still have biological implications. Here we show that when sequencing RNA fragments from one end, as is the case on most platforms, an oscillation in the read count is observed at the other end. We further show that these oscillations can be well described by Kolmogorov's 1941 broken stick model. We investigate how the model can be used to improve predictions of gene ends (3' transcript ends), but conclude that with present data the improvement is only marginal. The results highlight subtle effects in high-throughput transcriptomics experiments which do not have a biological origin, but which may still be used to obtain biological information.
Subject headings
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
Keyword
- Modelling
- Artefacts
- RNAseq
- Log-normal distribution
- Broken stick
- 3'end of transcripts
- SRA - Molecular Bioscience
- SRA - Molekylär biovetenskap
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database