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- Branca, Rui M. M.Karolinska Institutet (author)
HiRIEF LC-MSMS enables deep proteome coverage and unbiased proteogenomics
- Article/chapterEnglish2014
Publisher, publication year, extent ...
- 2014
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:kth-140375
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-140375URI
- https://doi.org/10.1038/NMETH.2732DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:128003993URI
- https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-100102URI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
Classification
Notes
- QC 20140123
- AuthorCount:9;
- We present a liquid chromatography-mass spectrometry (LC-MSMS)-based method permitting unbiased (gene prediction-independent) genome-wide discovery of protein-coding loci in higher eukaryotes. Using high-resolution isoelectric focusing (HiRIEF) at the peptide level in the 3.7-5.0 pH range and accurate peptide isoelectric point (pI) prediction, we probed the six-reading-frame translation of the human and mouse genomes and identified 98 and 52 previously undiscovered protein-coding loci, respectively. The method also enabled deep proteome coverage, identifying 13,078 human and 10,637 mouse proteins.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- Tandem Mass-Spectra
- Peptide Identification
- Human Genome
- Gene
- Fractionation
- Spectrometry
- Pseudogenes
- Annotation
- Landscape
- Dynamics
Added entries (persons, corporate bodies, meetings, titles ...)
- Orre, Lukas M.Karolinska Institutet (author)
- Johansson, Henrik J.Karolinska Institutet (author)
- Granholm, ViktorStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)(Swepub:su)vigr7481 (author)
- Huss, MikaelStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)(Swepub:su)mihu5153 (author)
- Perez-Bercoff, Åsa (author)
- Forshed, JennyKarolinska Institutet (author)
- Käll, LukasKTH,Genteknologi,Science for Life Laboratory, SciLifeLab,SeRC - Swedish e-Science Research Centre(Swepub:kth)u1gqsept (author)
- Lehtiö, JanneKarolinska Institutet (author)
- Karolinska InstitutetInstitutionen för biokemi och biofysik (creator_code:org_t)
Related titles
- In:Nature Methods11:1, s. 59-1548-70911548-7105
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