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HiRIEF LC-MSMS enab...
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Branca, Rui M. M.Karolinska Institutet
(author)
HiRIEF LC-MSMS enables deep proteome coverage and unbiased proteogenomics
- Article/chapterEnglish2014
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LIBRIS-ID:oai:DiVA.org:kth-140375
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-140375URI
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https://doi.org/10.1038/NMETH.2732DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:128003993URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-100102URI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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QC 20140123
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AuthorCount:9;
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We present a liquid chromatography-mass spectrometry (LC-MSMS)-based method permitting unbiased (gene prediction-independent) genome-wide discovery of protein-coding loci in higher eukaryotes. Using high-resolution isoelectric focusing (HiRIEF) at the peptide level in the 3.7-5.0 pH range and accurate peptide isoelectric point (pI) prediction, we probed the six-reading-frame translation of the human and mouse genomes and identified 98 and 52 previously undiscovered protein-coding loci, respectively. The method also enabled deep proteome coverage, identifying 13,078 human and 10,637 mouse proteins.
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Orre, Lukas M.Karolinska Institutet
(author)
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Johansson, Henrik J.Karolinska Institutet
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Granholm, ViktorStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)(Swepub:su)vigr7481
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Huss, MikaelStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)(Swepub:su)mihu5153
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Perez-Bercoff, Åsa
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Forshed, JennyKarolinska Institutet
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Käll, LukasKTH,Genteknologi,Science for Life Laboratory, SciLifeLab,SeRC - Swedish e-Science Research Centre(Swepub:kth)u1gqsept
(author)
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Lehtiö, JanneKarolinska Institutet
(author)
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Karolinska InstitutetInstitutionen för biokemi och biofysik
(creator_code:org_t)
Related titles
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In:Nature Methods11:1, s. 59-1548-70911548-7105
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