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Affinity ligands fo...
Affinity ligands for industrial protein purification
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Linhult, M. (author)
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Gulich, S. (author)
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- Hober, Sophia (author)
- KTH,Proteomik
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(creator_code:org_t)
- Bentham Science Publishers Ltd. 2005
- 2005
- English.
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In: Protein peptide letters. - : Bentham Science Publishers Ltd.. - 0929-8665 .- 1875-5305. ; 12:4, s. 305-310
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.2...
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Abstract
Subject headings
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- Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.
Keyword
- Affinity chromatography
- cleaning-in-place (CIP)
- deamidation
- protein A
- protein G
- protein engineering
- stabilization
- escherichia-coli
- synthetic ligand
- deamidation
- chromatography
- peptides
- residues
- stability
- design
- immobilization
- mutagenesis
Publication and Content Type
- ref (subject category)
- for (subject category)
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