Scanning inverse fluorescence correlation spectroscopy

• Scanning Inverse Fluorescence Correlation Spectroscopy (siFCS) is introduced to determine the absolute size of nanodomains on surfaces. We describe here equations for obtaining the domain size from cross-and auto-correlation functions, measurement simulations which enabled testing of these equations, and measurements on model surfaces mimicking membranes containing nanodomains. Using a confocal microscope of 270 nm resolution the size of 250 nm domains were estimated by siFCS to 257 +/- 12 nm diameter, and 40 nm domains were estimated to 65 +/- 26 nm diameter. Applications of siFCS for sizing of nanodomains and protein clusters in cell membranes are discussed.

Subject headings

NATURVETENSKAP  -- Biologi -- Biofysik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biophysics (hsv//eng)

Keyword

Image Correlation Spectroscopy

Cross-Correlation Spectroscopy

Stimulated-Emission

Microscopy

Nanoscopy

Proteins

Cells

Limit

Biological Physics

Biologisk fysik

Publication and Content Type

ref (subject category)

art (subject category)