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Differentiation of ...
Differentiation of normal and cancer cells induced by sulfhydryl reduction : biochemical and molecular mechanisms
- Article/chapterEnglish2005
Publisher, publication year, extent ...
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2005-05-27
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Springer Science and Business Media LLC,2005
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-15035
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-15035URI
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https://doi.org/10.1038/sj.cdd.4401663DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20100525
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We examined the morphological, biochemical and molecular outcome of a nonspecific sulfhydryl reduction in cells, obtained by supplementation of N-acetyl-L-cysteine (NAC) in a 0.1-10 mM concentration range. In human normal primary keratinocytes and in colon and ovary carcinoma cells we obtained evidences for: (i) a dose-dependent inhibition of proliferation without toxicity or apoptosis; (ii) a transition from a proliferative mesenchymal morphology to cell-specific differentiated structures; (iii) a noticeable increase in cell-cell and cell-substratum junctions; (iv) a relocation of the oncogenic beta-catenin at the cell-cell junctions; (v) inhibition of microtubules aggregation; (vi) upregulation of differentiation-related genes including p53, heat shock protein 27 gene, N-myc downstream-regulated gene 1, E-cadherin, and down-regulation of cyclooxygenase-2; (vii) inhibition of c-Src tyrosine kinase. In conclusion, a thiol reduction devoid of toxicity as that operated by NAC apparently leads to terminal differentiation of normal and cancer cells through a pleiade of converging mechanisms, many of which are targets of the recently developed differentiation therapy.
Subject headings and genre
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colon carcinoma
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cyclooxygenase-2
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junctions
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ovary carcinoma
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proliferation
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c-Src
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redox regulation
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src kinase
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colorectal-cancer
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n-acetylcysteine
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colon-cancer
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dna-damage
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stem-cells
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gene
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apoptosis
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cytoskeleton
Added entries (persons, corporate bodies, meetings, titles ...)
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Brunelli, R.
(author)
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Bracci-Laudiero, L.
(author)
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Greco, G.
(author)
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Gustafsson, A. C.
(author)
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Krasnowska, E. K.
(author)
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Lundeberg, JoakimKTH,Genteknologi(Swepub:kth)u1qkn9kw
(author)
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Lundeberg, T.
(author)
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Pittaluga, E.
(author)
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Romano, M. C.
(author)
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Serafino, A.
(author)
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KTHGenteknologi
(creator_code:org_t)
Related titles
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In:Cell Death and Differentiation: Springer Science and Business Media LLC12:10, s. 1285-12961350-90471476-5403
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Parasassi, T.
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Brunelli, R.
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Bracci-Laudiero, ...
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Greco, G.
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Gustafsson, A. C ...
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Krasnowska, E. K ...
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Lundeberg, Joaki ...
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Lundeberg, T.
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Pittaluga, E.
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Romano, M. C.
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Serafino, A.
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Cell Death and D ...
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Royal Institute of Technology