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- Gunnarson, E.Karolinska Institutet (author)
Lead induces increased water permeability in astrocytes expressing aquaporin 4
- Article/chapterEnglish2005
Publisher, publication year, extent ...
- Elsevier BV,2005
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:kth-15185
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-15185URI
- https://doi.org/10.1016/j.neuroscience.2005.07.027DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:1947915URI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- QC 20150724
- The water channel aquaporin 4 (AQP4) is abundantly expressed in astrocytes. There is now compelling evidence that AQP4 may contribute to an unfavorable course in brain edema. Acute lead intoxication is a condition that causes brain damage preceded by brain edema. Here we report that lead increases AQP4 water permeability (P-f) in astrocytes. A rat astrocyte cell line that does not express aquaporin 4 was transiently transfected with aquaporin 4 tagged with green fluorescent protein (GFP). Using confocal laser scanning microscopy we measured water permeability in these cells and in AQP4-negative cells located on the same plate. AQP4-expressing astrocytes had a three-fold higher water permeability than astrocytes not expressing AQP4. Lead exposure induced a significant, 40%, increase in water permeability in astrocytes expressing AQP4, but had no effect on Pf in astrocytes not expressing AQP4. The increase in water permeability persisted after lead washout, while treatment with a lead chelator, meso-2,3-dimercaptosuccinic acid, abolished the lead-induced increase in Pf. The effect of lead was attenuated in the presence of a calcium (Ca2+)/ calmodulin-dependent protein kinase 11 (CaMKII) inhibitor, but not in the presence of a protein kinase C inhibitor. In cells expressing AQP4 where the consensus site for CaMKII phosphorylation was mutated, lead failed to increase water permeability. Lead exposure also increased Pf in rat astroglial cells in primary culture, which express endogenous AQP4. Lead had no effect on Pf in astrocytes transfected with aquaporin 3. In situ hybridization studies on rat brain after oral lead intake for three days showed no change in distribution of AQP4 mRNA. It is suggested that lead-triggered stimulation of water transport in AQP4-expressing astrocytes may contribute to the pathology of acute lead intoxication.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Neurovetenskaper hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Neurosciences hsv//eng
- NATURVETENSKAP Biologi Cellbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Cell Biology hsv//eng
- glial cells
- lead
- toxicity
- water channel
- confocal microscopy
- primary culture
- protein-kinase-c
- brain edema
- rat-brain
- messenger-rna
- membrane
- domains
- channel protein
- cells
- encephalopathy
- mice
- mislocalization
Added entries (persons, corporate bodies, meetings, titles ...)
- Axehult, G. (author)
- Baturina, G. (author)
- Zelenin, S.Karolinska Institutet (author)
- Zelenina, MarinaKarolinska Institutet,KTH,Cellens fysik(Swepub:kth)u14w09k8 (author)
- Aperia, A.Karolinska Institutet (author)
- Karolinska InstitutetCellens fysik (creator_code:org_t)
Related titles
- In:Neuroscience: Elsevier BV136:1, s. 105-1140306-45221873-7544
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