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Distinguishing xylo...
Distinguishing xyloglucanase activity in endo-β(1 → 4)glucanases
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- Eklof, Jens M. (author)
- KTH,Glykovetenskap,Michael Smith Laboratories, Department of Chemistry, University of British Columbia, Canada
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- Ruda, Marcus C. (author)
- SweTree Technologies, P.O. Box 4095, Umeå, Sweden
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- Brumer, Harry (author)
- KTH,Glykovetenskap,Michael Smith Laboratories, Department of Chemistry, University of British Columbia, Canada
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(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- English.
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In: Methods in Enzymology. - : Elsevier BV. - 0076-6879 .- 1557-7988. - 9780124159310 ; 510, s. 97-120
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The ability of beta-glucanases to cleave xyloglucans, a family of highly decorated beta-glucans ubiquitous in plant biomass, has traditionally been overlooked in functional biochemical studies. An emerging body of data indicates, however, that a spectrum of xyloglucan specificity resides in diverse glycoside hydrolases from a range of carbohydrate-active enzyme families including classic "cellulase" families. This chapter outlines a series of enzyme kinetic and product analysis methods to establish degrees of xyloglucan specificity and modes of action of glycosidases emerging from enzyme discovery projects.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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