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Association of rena...
Abstract
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- Na,K-ATPase transports Na+ and K+ across cell membranes and consists of alpha- and beta-subunits. Na,K-ATPase also associates with small FXYD proteins that regulate the activity of the pump. We have used cryoelectron microscopy of two-dimensional crystals including data to 8 A resolution to determine the three-dimensional (3-D) structure of renal Na,K-ATPase containing FXYD2, the gamma-subunit. A homology model for the a- subunit was calculated from a Ca2+-ATPase structure and used to locate the additional beta- and gamma-subunits present in the 3-D map of Na,K-ATPase. Based on the 3-D map, the beta- subunit is located close to transmembrane helices M8 and M10 and the gamma-subunit is adjacent to helices M2 and M9 of the alpha-subunit.
Keyword
- Na
- K-ATPase
- cryoelectron microscopy
- gamma-subunit
- beta-subunit
- FXYD2
- calcium-pump
- crystal-structure
- 3-dimensional structure
- sarcoplasmic-reticulum
- cross-links
- atpase
- resolution
- binding
- domain
- ca2+-atpase
Publication and Content Type
- ref (subject category)
- art (subject category)
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