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Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets
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- Joensson, Haakan (author)
- KTH,Nanobioteknologi
- Samuels, M. L. (author)
- Brouzes, E. R. (author)
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- Medkova, M. (author)
- Link, D. R. (author)
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- (creator_code:org_t)
- Chemical and Biological Microsystems Society, 2008
- 2008
- English.
- In: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. - : Chemical and Biological Microsystems Society. ; , s. 1287-1289
- Conference paper (peer-reviewed)
Abstract
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- We have developed a novel microfluidic droplet based assay for analysis of low expressed cell surface proteins on individual cells at rates of hundreds of cells/s by antibody coupled enzymatic amplification in monodisperse droplets [1]. Here we expand the method to include concurrent analysis of multiple populations of single cells. We report the validation of the method by analyzing the human monocytic cell line U937 for two low expressed markers, CCR5 and CD19. Comparing our method to standard flow cytometry, we demonstrate increased peak separation, which should allow sorting by these low expressed biomarkers unavailable to flow cytometry.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Antibodies
- Cell surface biomarkers
- Droplets
- Enzymes
Publication and Content Type
- ref (subject category)
- kon (subject category)
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- Joensson, Haakan
- Samuels, M. L.
- Brouzes, E. R.
- Medkova, M.
- Uhlén, Mathias
- Andersson Svahn, ...
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- Link, D. R.
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- Royal Institute of Technology
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