Centrifugal microfluidic system for rapid, low-cost HIV diagnosis : CD4+ T-cell counting using an integrated DVD platform

• HIV is a pandemic that currently threatens over 33 million lives worldwide and HIV/AIDS remains one of the major causes of death globally. The continued monitoring of the CD4+ T-lymphocytes count in HIV patients is necessary for proper treatment, although this testing is too expensive and complex for limited resource settings. We report on a novel integrated centrifugal (CD) microfluidic system for rapid and low-cost HIV diagnosis through automated counting of CD4+ T-cells for point-of-care applications. We demonstrate the integrated T-cell immunocapture and detection mechanism using a novel system comprised of a modified commercial DVD drive and polymer disc.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology (hsv//eng)

Keyword

Centrifugal microfluidics

HIV diagnostics

Immunocapture

Publication and Content Type

ref (subject category)

kon (subject category)