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Bioactive Surface M...
Bioactive Surface Modification of Metal Oxides via Catechol-Bearing Modular Peptides : Multivalent-Binding, Surface Retention, and Peptide Bioactivity
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Tang, Wen (author)
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Policastro, Gina M. (author)
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- Hua, Geng (author)
- University of Akron, United States
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Guo, Kai (author)
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Zhou, Jinjun (author)
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Wesdemiotis, Chrys (author)
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Doll, Gary L. (author)
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Becker, Matthew L. (author)
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(creator_code:org_t)
- 2014-11-07
- 2014
- English.
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In: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 136:46, s. 16357-16367
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- A series of multivalent dendrons containing a bioactive osteogenic growth peptide (OGP) domain and surface-binding catechol domains were obtained through solid phase synthesis, and their binding affinity to hydroxyapatite, TiO2, ZrO2, CeO2, Fe3O4 and gold was characterized using a quartz crystal microbalance with dissipation (QCM-d). Using the distinct difference in binding affinity of the bioconjugate to the metal oxides, TiO2-coated glass slides were selectively patterned with bioactive peptides. Cell culture studies demonstrated the bioavailability of the OGP and that OGP remained on the surface for at least 2 weeks under in vitro cell culture conditions. Bone sialoprotein (BSP) and osteocalcein (OCN) markers were upregulated 3-fold and 60-fold, respectively, relative to controls at 21 days. Similarly, 3-fold more calcium was deposited using the OGP tethered dendron compared to TiO2. These catechol-bearing dendrons provide a fast and efficient method to functionalize a wide range of inorganic materials with bioactive peptides and have the potential to be used in coating orthopaedic implants and fixation devices.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Bearbetnings-, yt- och fogningsteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Manufacturing, Surface and Joining Technology (hsv//eng)
Keyword
- Binding energy
- Bioactive glass
- Biochemistry
- Cell culture
- Dendrimers
- Phenols
- Quartz
- Quartz crystal microbalances
- Synthesis (chemical)
- Titanium dioxide
- Binding affinities
- Bioactive peptides
- Bone sialoproteins
- Inorganic materials
- Orthopaedic implants
- Osteogenic growth peptides
- Quartz crystal microbalance with dissipation
- Solid phase synthesis
Publication and Content Type
- ref (subject category)
- art (subject category)
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