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Structural evidence...
Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases : Biological implications for cell wall metabolism
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- Baumann, Martin J. (author)
- KTH,Skolan för bioteknologi (BIO)
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- Eklöf, Jens M. (author)
- KTH,Glykovetenskap
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Michel, Gurvan (author)
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- Kallas, Åsa M. (author)
- KTH,Skolan för bioteknologi (BIO)
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- Teeri, Tuula T. (author)
- KTH,Glykovetenskap
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Czjzek, Mirjam (author)
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- Brumer, Harry (author)
- KTH,Glykovetenskap
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(creator_code:org_t)
- 2007-06-08
- 2007
- English.
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In: The Plant Cell. - : Oxford University Press (OUP). - 1040-4651 .- 1532-298X. ; 19:6, s. 1947-1963
- Related links:
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http://www.plantcell...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure-function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo-transglycosylase Ptt-XET16-34 from hybrid aspen ( Populus tremula 3 Populus tremuloides). Production of the loop deletion variant Tm-NXG1-Delta YNIIG yielded an enzyme that was structurally similar to Ptt- XET16-34 and had a greatly increased transglycosylation: hydrolysis ratio. Comprehensive bioinformatic analyses of XTH gene products, together with detailed kinetic data, strongly suggest that xyloglucanase activity has evolved as a gain of function in an ancestral GH16 XET to meet specific biological requirements during seed germination, fruit ripening, and rapid wall expansion.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- tropaeolum-majus l
- germinated nasturtium seeds
- glycoside hydrolases
- expression analysis
- kappa-carrageenase
- sequence alignment
- crystal-structures
- pichia-pastoris
- hybrid aspen
- endotransglycosylase
- Biochemistry
- Biokemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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