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Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases : Biological implications for cell wall metabolism

Baumann, Martin J. (author)
KTH,Skolan för bioteknologi (BIO)
Eklöf, Jens M. (author)
KTH,Glykovetenskap
Michel, Gurvan (author)
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Kallas, Åsa M. (author)
KTH,Skolan för bioteknologi (BIO)
Teeri, Tuula T. (author)
KTH,Glykovetenskap
Czjzek, Mirjam (author)
Brumer, Harry (author)
KTH,Glykovetenskap
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 (creator_code:org_t)
2007-06-08
2007
English.
In: The Plant Cell. - : Oxford University Press (OUP). - 1040-4651 .- 1532-298X. ; 19:6, s. 1947-1963
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by x-ray crystallography. Key structural features that modulate the relative rates of substrate hydrolysis to transglycosylation in the GH16 xyloglucan-active enzymes were identified by structure-function studies of the recombinantly expressed enzymes in comparison with data for the strict xyloglucan endo-transglycosylase Ptt-XET16-34 from hybrid aspen ( Populus tremula 3 Populus tremuloides). Production of the loop deletion variant Tm-NXG1-Delta YNIIG yielded an enzyme that was structurally similar to Ptt- XET16-34 and had a greatly increased transglycosylation: hydrolysis ratio. Comprehensive bioinformatic analyses of XTH gene products, together with detailed kinetic data, strongly suggest that xyloglucanase activity has evolved as a gain of function in an ancestral GH16 XET to meet specific biological requirements during seed germination, fruit ripening, and rapid wall expansion.

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

tropaeolum-majus l
germinated nasturtium seeds
glycoside hydrolases
expression analysis
kappa-carrageenase
sequence alignment
crystal-structures
pichia-pastoris
hybrid aspen
endotransglycosylase
Biochemistry
Biokemi

Publication and Content Type

ref (subject category)
art (subject category)

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