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Single-cell charact...
Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
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- Tauriainen, Johanna (author)
- Karolinska Institutet
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- Gustafsson, Karin (author)
- KTH,Tillämpad fysik,Science for Life Laboratory, SciLifeLab
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- Göthlin, Mårten (author)
- KTH,Tillämpad fysik,Science for Life Laboratory, SciLifeLab
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Gertow, Jens (author)
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- Buggert, Marcus (author)
- Karolinska Institutet
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- Frisk, Thomas W. (author)
- KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
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- Karlsson, Annika C. (author)
- Karolinska Institutet
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- Uhlin, Michael (author)
- Karolinska Institutet
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- Önfelt, Björn (author)
- KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab,Karolinska Institutet, Sweden
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(creator_code:org_t)
- 2015-04-28
- 2015
- English.
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In: Frontiers in Immunology. - : Frontiers Media SA. - 1664-3224. ; 6
- Related links:
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https://doi.org/10.3...
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https://www.frontier...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells, and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation, and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division, and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.
Subject headings
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Keyword
- T cell
- IL-2
- IL-7
- microscopy
- fluorescence
- microchip
- single-cell analysis
- live-cell imaging
Publication and Content Type
- ref (subject category)
- art (subject category)
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