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Studies on an on/of...
Studies on an on/off-switchable immunosensor for troponin T
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- Ashaduzzaman, M. (author)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska fakulteten,University of Dhaka, Bangladesh
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- Anto Antony, A. (author)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska fakulteten
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- Arul Murugan, Natarajan (author)
- KTH,Teoretisk kemi och biologi,Virtual Laboratory for Molecular Probes,Royal Institute Technology, Sweden
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- Deshpande, S. R. (author)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska fakulteten
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- Turner, Anthony (author)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska fakulteten
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- Tiwari, Ashutosh (author)
- Linköpings universitet,Biosensorer och bioelektronik,Tekniska fakulteten,Tekidag AB, UCS, S-58330 Linkoping, Sweden
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- English.
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In: Biosensors & bioelectronics. - : Elsevier BV. - 0956-5663 .- 1873-4235. ; 73
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Regeneration is a key goal in the design of immunosensors. In this study, we report the temperature-regulated interaction of N-isopropylacrylamide (PNIPAAm) functionalised cardiac troponin T (cTnT) with anti-cTnT. Covalently bonded PNIPAAm on an anti-cTnT bioelectrode showed on/off-switchability, regeneration capacity and temperature triggered sensitivity for cTnT. Above the lower critical solution temperature (LCST), PNIPAAm provides a liphophilic microenvironment with specific volume reduction at the bioelectrode surface, making available binding space for cTnT, and facilitating analyte recognition. Computational studies provide details about the structural changes occurring at the electrode above and below the LCST. Furthermore, free energies associated with the binding of cTnT with PNIPAAm at 25 (δGcoil=-6.0Kcal/mole) and 37°C (δGglobular=-41.0kcal/mole) were calculated to elucidate the interaction and stability of the antigen-antibody complex. The responsiveness of such assemblies opens the way for miniaturised, smart immuno-technologies with 'built-in' programmable interactions of antigen-antibody upon receiving stimuli.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Keyword
- On/off-switching
- Programmable bioelectronics
- Smart immunosensors
- Troponin T
Publication and Content Type
- ref (subject category)
- art (subject category)
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