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Detection and quant...
Detection and quantitative estimation of spurious double stranded DNA formation during reverse transcription in bateria using tagRNA-seq
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- Innocenti, Nicolas, 1986- (författare)
- KTH,Beräkningsbiologi, CB,Computational Biological Physics, CBP
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- Repoila, Francis (författare)
- INRA, UMR1319 Micalis, Domaine de Vilvert, F-78352, Jouy-en-Josas, France}\affiliation{AgroParisTech, UMR Micalis, Domaine de Vilvert, F-78350, Jouy-en-Josas, France
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- Aurell, Erik, 1961- (författare)
- KTH,Beräkningsbiologi, CB,Computational Biological Physics, CBP
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(creator_code:org_t)
- 2015-07-15
- 2015
- Engelska.
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Ingår i: RNA Biology. - : Taylor & Francis. - 1547-6286 .- 1555-8584.
- Relaterad länk:
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visa fler...
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https://kth.diva-por... (primary) (Raw object)
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https://www.tandfonl...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Standard RNA-seq has a well know tendency to generate "ghost" antisense reads due to formation of spurious second strand cDNA in the sequencing process. We recently reported on a novel variant of RNA-seq coined "tagRNA-seq" introduced for the purpose of distinguishing primary from processed transcripts in bacteria. Incidentally, the additional information provided by the tag is also very suitable for detection of true anti-sense RNA transcripts and quantification of spurious antisense signals in a sample. We briefly explain how to perform such a detection and illustrate on previously published datasets.
Ämnesord
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- tagRNA-seq
- spurious second strand cDNA
- antisense RNA
- complementary DNA
- transcriptome
- transcript discovery
- Bioteknologi
- Biotechnology
- Biological Physics
- Biologisk fysik
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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