Extensive weight loss reveals distinct gene expression changes in human subcutaneous and visceral adipose tissue

Mardinoglu, AdilKTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Chalmers University of Technology, Sweden,Chalmers tekniska högskola (author)

QC 20151104
Weight loss has been shown to significantly improve Adipose tissue (AT) function, however changes in AT gene expression profiles particularly in visceral AT (VAT) have not been systematically studied. Here, we tested the hypothesis that extensive weight loss in response to bariatric surgery (BS) causes AT gene expression changes, which may affect energy and lipid metabolism, inflammation and secretory function of AT. We assessed gene expression changes by whole genome expression chips in AT samples obtained from six morbidly obese individuals, who underwent a two step BS strategy with sleeve gastrectomy as initial and a Roux-en-Y gastric bypass as second step surgery after 12 +/- 2 months. Global gene expression differences in VAT and subcutaneous (S) AT were analyzed through the use of genome-scale metabolic model (GEM) for adipocytes. Significantly altered gene expressions were PCR-validated in 16 individuals, which also underwent a two-step surgery intervention. We found increased expression of cell death-inducing DFFA-like effector a (CIDEA), involved in formation of lipid droplets in both fat depots in response to significant weight loss. We observed that expression of the genes associated with metabolic reactions involved in NAD+, glutathione and branched chain amino acid metabolism are significantly increased in AT depots after surgery-induced weight loss.

Heiker, John T.Universität Leipzig,Leipzig University (author)
Gaertner, DanielStadtischen Klinikum Karlsruhe (author)
Björnson, Elias,1988Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)eliasb (author)
Schoen, Michael R.Stadtischen Klinikum Karlsruhe (author)
Flehmig, GesineUniversität Leipzig,Leipzig University (author)
Kloeting, Nora (author)
Krohn, KnutUniversitatsklinikum Leipzig und Medizinische Fakultat,Leipzig University Hospital (author)
Fasshauer, MathiasUniversität Leipzig,Leipzig University (author)
Stumvoll, MichaelUniversität Leipzig,Leipzig University (author)
Nielsen, JensKTH,Genteknologi,Science for Life Laboratory, SciLifeLab,Chalmers University of Technology, Sweden,Chalmers tekniska högskola(Swepub:cth)nielsenj (author)
Blueher, MatthiasUniversität Leipzig,Leipzig University (author)
KTHProteomik och nanobioteknologi (creator_code:org_t)

By the author/editor: Mardinoglu, Adil; Heiker, John T.; Gaertner, Daniel; Björnson, Elias, ...; Schoen, Michael ...; Flehmig, Gesine; show more...; Kloeting, Nora; Krohn, Knut; Fasshauer, Mathi ...; Stumvoll, Michae ...; Nielsen, Jens; Blueher, Matthia ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Bioinformatics a ...

By the university: Royal Institute of Technology; Chalmers University of Technology

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