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Electrochemical mic...
Abstract
Ämnesord
Stäng
- We present an electrochemical lab-on-a-chip (LOC) platform for the simultaneous detection of up to four different analytes. The possibility to separately immobilize different assays in a channel network, without active valves, was successfully demonstrated using a model assay linked to glucose oxidase. This enables the detection of various analytes even with different assay formats. For the assay immobilization, the channel surface, made out of dry film photoresist (DFR), could be activated by means of EDC/NHS-linker chemistry and used for the covalent binding of primary amines. Cross-sensitivity due to diffusion within the channel network could be experimentally excluded.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Nyckelord
- Immunoassay
- microfluidics
- electrochemical biosensor
- dry-film photoresist
- lab-on-a-chip
- Multi-analyte
- Assays
- Bioassay
- Glucose oxidase
- Glucose sensors
- Lab-on-a-chip
- Photoresists
- Channel surface
- Covalent binding
- Cross sensitivity
- Dry-film photoresists
- Electrochemical biosensor
- Microfluidic platforms
- Multi-analytes
- Simultaneous detection
- Microfluidics
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)