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Annular dark field ...
Annular dark field transmission electron microscopy for protein structure determination
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- Koeck, Philip J. B. (author)
- Karolinska Institutet,KTH,Strukturell bioteknik,Department of Biosciences and Nutrition, Novum, Huddinge, Sweden
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(creator_code:org_t)
- Elsevier, 2016
- 2016
- English.
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In: Ultramicroscopy. - : Elsevier. - 0304-3991 .- 1879-2723. ; 161, s. 98-104
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Abstract
Subject headings
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- Recently annular dark field (ADF) transmission electron microscopy (TEM) has been advocated as a means of recording images of biological specimens with better signal to noise ratio (SNR) than regular bright field images. I investigate whether and how such images could be used to determine the three-dimensional structure of proteins given that an ADF aperture with a suitable pass-band can be manufactured and used in practice. I develop an approximate theory of ADF-TEM image formation for weak amplitude and phase objects and test this theory using computer simulations. I also test whether these simulated images can be used to calculate a three-dimensional model of the protein using standard software and discuss problems and possible ways to overcome these.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk bildbehandling (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Image Processing (hsv//eng)
Keyword
- Annular dark field aperture
- Linear imaging
- Phase contrast transfer function
- Projection approximation
- Resolution
- Transmission electron microscopy
Publication and Content Type
- ref (subject category)
- art (subject category)
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