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Detection and Analy...
Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets
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- Jönsson, Håkan (author)
- KTH,Nanobioteknologi
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Samuels, Michael L. (author)
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Brouzes, Eric R. (author)
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Medkova, Martina (author)
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- Uhlén, Mathias (author)
- KTH,Proteomik
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Link, Darren R. (author)
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- Andersson-Svahn, Helene (author)
- KTH,Nanobioteknologi
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(creator_code:org_t)
- Wiley, 2009
- 2009
- English.
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In: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 48:14, s. 2518-2521
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- droplets
- enzymatic amplification
- high-throughput screening
- microfluidics
- proteins
- polymerase-chain-reaction
- protein expression
- picoliter droplets
- single cells
- devices
- microdroplets
- encapsulation
- molecules
- therapy
- gene
Publication and Content Type
- ref (subject category)
- art (subject category)
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