SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:kth-18433"
 

Search: onr:"swepub:oai:DiVA.org:kth-18433" > Analysis of nasturt...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Analysis of nasturtium TmNXG1 complexes by crystallography and molecular dynamics provides detailed insight into substrate recognition by family GH16 xyloglucan endo-transglycosylases and endo-hydrolases

Mark, Pekka (author)
KTH,Glykovetenskap
Baumann, Martin J. (author)
KTH,Glykovetenskap
Eklöf, Jens M. (author)
KTH,Glykovetenskap
show more...
Gullfot, Fredrika (author)
KTH,Glykovetenskap
Michel, Gurvan (author)
Kallas, Åsa M. (author)
KTH,Glykovetenskap
Teeri, Tuula T. (author)
KTH,Glykovetenskap
Brumer, Harry (author)
KTH,Glykovetenskap
Czjzek, Mirjam (author)
show less...
 (creator_code:org_t)
Wiley, 2009
2009
English.
In: Proteins. - : Wiley. - 0887-3585 .- 1097-0134. ; 75:4, s. 820-836
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • Reorganization and degradation of the wall crosslinking and seed storage polysaccharide xyloglucan by glycoside hydrolase family 16 (GH16) endo-transglycosylases and hydrolases are crucial to the growth of the majority of land plants, affecting processes as diverse as germination, morphogenesis, and fruit ripening. A high-resolution, three-dimensional structure of a nasturtium (Tropaeolum majus) endo-xyloglucanase loop mutant, TmNXG1-Delta YNIIG, with an ohgosaccharide product bound in the negative active-site subsites, has been solved by X-ray crystallography. Comparison of this novel complex to that of the strict xyloglucan endotransglycosylase PttXET16-34 from hybrid aspen (Populus tremula x tremuloides), previously solved with a xylogluco-oligosaccharide bound in the positive subsites, highlighted key protein structures that affect the disparate catalytic activities displayed by these closely related enzymes. Combination of these "partial" active-site complexes through molecular dynamics simulations in water allowed modeling of wild-type TmNXG1, TmNXG1-Delta YNIIG, and wild-type PttXET16-34 in complex with a xyloglucan octadecasaccharide spanning the entire catalytic cleft. A comprehensive analysis of these full-length complexes underscored the importance of various loops lining the active site. Subtle differences leading to a tighter hydrogen bonding pattern on the negative (glycosyl donor) binding subsites, together with loop flexibility on the positive (glycosyl acceptor) binding subsites appear to favor hydrolysis over transglycosylation in GH16 xyloglucan-active enzymes.

Keyword

xyloglucan
nasturtium seedlings
cell wall polysaccharides
crystal
structure
molecular dynamics
cell-walls
glycoside hydrolases
crystal-structures
oligosaccharides
endotransglycosylase
nomenclature
acceptor
perspectives
degradation
cotyledons

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

  • Proteins (Search for host publication in LIBRIS)

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view