Search: onr:"swepub:oai:DiVA.org:kth-185604" >
Microchip screening...
Microchip screening Platform for single cell assessment of NK cell cytotoxicity
-
- Guldevall, Karolin (author)
- KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
-
- Brandt, Ludwig (author)
- KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
-
- Forslund, Elin (author)
- KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab,Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Sweden
-
show more...
-
Olofsson, Karl (author)
-
- Frisk, Thomas W. (author)
- KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
-
- Olofsson, Per E. (author)
- KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
-
- Gustafsson, Karin (author)
- KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
-
- Manneberg, Otto (author)
- KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
-
- Vanherberghen, Bruno (author)
- KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
-
- Brismar, Hjalmar (author)
- Karolinska Institutet,KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
-
- Karre, Klas (author)
- Karolinska Institutet
-
- Uhlin, Michael (author)
- Karolinska Institutet
-
- Önfelt, Björn (author)
- Karolinska Institutet,KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab
-
show less...
-
(creator_code:org_t)
- 2016-04-05
- 2016
- English.
-
In: Frontiers in Immunology. - : FRONTIERS MEDIA SA. - 1664-3224. ; 7
- Related links:
-
https://doi.org/10.3...
-
show more...
-
https://www.frontier...
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Here, we report a screening platform for assessment of the cytotoxic potential of individual natural killer (NK) cells within larger populations. Human primary NK cells were distributed across a silicon-glass microchip containing 32,400 individual microwells loaded with target cells. Through fluorescence screening and automated image analysis, the numbers of NK and live or dead target cells in each well could be assessed at different time points after initial mixing. Cytotoxicity was also studied by time-lapse live-cell imaging in microwells quantifying the killing potential of individual NK cells. Although most resting NK cells (approximate to 75%) were non-cytotoxic against the leukemia cell line K562, some NK cells were able to kill several (>= 3) target cells within the 12-h long experiment. In addition, the screening approach was adapted to increase the chance to find and evaluate serial killing NK cells. Even if the cytotoxic potential varied between donors, it was evident that a small fraction of highly cytotoxic NK cells were responsible for a substantial portion of the killing. We demonstrate multiple assays where our platform can be used to enumerate and characterize cytotoxic cells, such as NK or T cells. This approach could find use in clinical applications, e.g., in the selection of donors for stem cell transplantation or generation of highly specific and cytotoxic cells for adoptive immunotherapy.
Subject headings
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Keyword
- NK cells
- cytotoxicity
- single cell analysis
- microchip
- screening
- microscopy
- fluorescence
- immune synapse
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Guldevall, Karol ...
-
Brandt, Ludwig
-
Forslund, Elin
-
Olofsson, Karl
-
Frisk, Thomas W.
-
Olofsson, Per E.
-
show more...
-
Gustafsson, Kari ...
-
Manneberg, Otto
-
Vanherberghen, B ...
-
Brismar, Hjalmar
-
Karre, Klas
-
Uhlin, Michael
-
Önfelt, Björn
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Immunology
- Articles in the publication
-
Frontiers in Imm ...
- By the university
-
Royal Institute of Technology
-
Karolinska Institutet