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Structural and Func...
Structural and Functional Analysis of Calcium Ion Mediated Binding of 5-Lipoxygenase to Nanodiscs
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- Balakrishnan Kumar, Ramakrishnan (author)
- Karolinska Institutet,KTH,Strukturell bioteknik,Department of Biosciences and Nutrition, Karolinska Institutet, Sweden
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- Zhu, Lin (author)
- KTH,Strukturell bioteknik,Department of Biosciences and Nutrition, Karolinska Institutet,
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- Idborg, Helena (author)
- Karolinska Institutet
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- Radmark, Olof (author)
- Karolinska Institutet
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- Jakobsson, Per-Johan (author)
- Karolinska Institutet
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Rinaldo-Matthis, Agnes (author)
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- Hebert, Hans (author)
- Karolinska Institutet,KTH,Strukturell bioteknik,Department of Biosciences and Nutrition, Karolinska Institutet,
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- Jegerschöld, Caroline (author)
- Karolinska Institutet,KTH,Strukturell bioteknik,Department of Biosciences and Nutrition, Karolinska Institutet,
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(creator_code:org_t)
- 2016-03-24
- 2016
- English.
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In: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 11:3
- Related links:
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https://doi.org/10.1...
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https://kth.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- An important step in the production of inflammatory mediators of the leukotriene family is the Ca2+ mediated recruitment of 5 Lipoxygenase (5LO) to nuclear membranes. To study this reaction in vitro, the natural membrane mimicking environment of nanodiscs was used. Nanodiscs with 10.5 nm inner diameter were made with the lipid POPC and membrane scaffolding protein MSP1E3D1. Monomeric and dimeric 5LO were investigated. Monomeric 5LO mixed with Ca2+ and nanodiscs are shown to form stable complexes that 1) produce the expected leukotriene products from arachidonic acid and 2) can be, for the first time, visualised by native gel electrophoresis and negative stain transmission electron micros-copy and 3) show a highest ratio of two 5LO per nanodisc. We interpret this as one 5LO on each side of the disc. The dimer of 5LO is visualised by negative stain transmission electron microscopy and is shown to not bind to nanodiscs. This study shows the advantages of nanodiscs to obtain basic structural information as well as functional information of a complex between a monotopic membrane protein and the membrane.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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