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  • Hamsten, C.Karolinska Institutet,Immunology and Allergy Unit; Department of Medicine Solna; Karolinska Institutet; and Karolinska University Hospital;Center for Inflammatory Diseases; Karolinska Institutet; Stockholm Sweden (author)

Protein profiles of CCL5, HPGDS, and NPSR1 in plasma reveal association with childhood asthma

  • Article/chapterEnglish2016

Publisher, publication year, extent ...

  • 2016-05-25
  • Blackwell Publishing,2016
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:kth-194276
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-194276URI
  • https://doi.org/10.1111/all.12927DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-306274URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:134264183URI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-491780URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • QC 20161024
  • Asthma is a common chronic childhood disease with many different phenotypes that need to be identified. We analyzed a broad range of plasma proteins in children with well-characterized asthma phenotypes to identify potential markers of childhood asthma. Using an affinity proteomics approach, plasma levels of 362 proteins covered by antibodies from the Human Protein Atlas were investigated in a total of 154 children with persistent or intermittent asthma and controls. After screening, chemokine ligand 5 (CCL5) hematopoietic prostaglandin D synthase (HPGDS) and neuropeptide S receptor 1 (NPSR1) were selected for further investigation. Significantly lower levels of both CCL5 and HPGDS were found in children with persistent asthma, while NPSR1 was found at higher levels in children with mild intermittent asthma compared to healthy controls. In addition, the protein levels were investigated in another respiratory disease, sarcoidosis, showing significantly higher NPSR1 levels in sera from sarcoidosis patients compared to healthy controls. Immunohistochemical staining of healthy tissues revealed high cytoplasmic expression of HPGDS in mast cells, present in stroma of both airway epithelia, lung as well as in other organs. High expression of NPSR1 was observed in neuroendocrine tissues, while no expression was observed in airway epithelia or lung. In conclusion, we have utilized a broad-scaled affinity proteomics approach to identify three proteins with altered plasma levels in asthmatic children, representing one of the first evaluations of HPGDS and NPSR1 protein levels in plasma.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Häggmark, Anna,1985-KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik,KTH Royal Inst Technol, Sch Biotechnol, SciLifeLab, Affin Prote, Stockholm, Sweden.,Affinity Proteomics; SciLifeLab; School of Biotechnology; KTH-Royal Institute of Technology; Stockholm Sweden(Swepub:kth)u1g5roxd (author)
  • Grundstrom, J.Karolinska Institutet,Immunology and Allergy Unit; Department of Medicine Solna; Karolinska Institutet; and Karolinska University Hospital (author)
  • Mikus, MariaKarolinska Institutet,KTH,Proteomik och nanobioteknologi,KTH Royal Inst Technol, Sch Biotechnol, SciLifeLab, Affin Prote, Stockholm, Sweden.,Karolinska Inst, Inst Environm Med, Stockholm, Sweden.;Soder Sjukhuset, Sachs Children & Youth Hosp, Stockholm, Sweden.,Karolinska Inst, Immunol & Allergy Unit, Dept Med Solna, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.,Affinity Proteomics; SciLifeLab; School of Biotechnology; KTH-Royal Institute of Technology; Stockholm Sweden,Institute of Environmental Medicine; Karolinska Institutet; Stockholm Sweden;Sachs’ Children and Youth Hospital; Södersjukhuset; Stockholm Sweden,Immunology and Allergy Unit; Department of Medicine Solna; Karolinska Institutet; and Karolinska University Hospital(Swepub:kth)u1qjoszn (author)
  • Lindskog, CeciliaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab,Cancerprecisionsmedicin,SciLifeLab; Department of Immunology; Genetics and Pathology; Uppsala University; Uppsala Sweden(Swepub:uu)cecli129 (author)
  • Konradsen, J. R.Karolinska Institutet,Astrid Lindgren Children's Hospital; Karolinska University Hospital; Stockholm Sweden;Department of Women's and Children's Health; Karolinska Institutet; Stockholm Sweden (author)
  • Eklund, A.Karolinska Institutet,Respiratory Medicine Unit; Department of Medicine Solna and CMM; Karolinska Institutet and Karolinska University Hospital; Stockholm Sweden (author)
  • Pershagen, G.Karolinska Institutet,Institute of Environmental Medicine; Karolinska Institutet; Stockholm Sweden (author)
  • Wickman, M. (author)
  • Grunewald, J.Karolinska Institutet,Respiratory Medicine Unit; Department of Medicine Solna and CMM; Karolinska Institutet and Karolinska University Hospital; Stockholm Sweden (author)
  • Melen, E.Karolinska Institutet,Institute of Environmental Medicine; Karolinska Institutet; Stockholm Sweden;Sachs’ Children and Youth Hospital; Södersjukhuset; Stockholm Sweden (author)
  • Hedlin, G.Karolinska Institutet,Astrid Lindgren Children's Hospital; Karolinska University Hospital; Stockholm Sweden;Department of Women's and Children's Health; Karolinska Institutet; Stockholm Sweden (author)
  • Nilsson, PeterKTH,Proteomik och nanobioteknologi,KTH Royal Inst Technol, Sch Biotechnol, SciLifeLab, Affin Prote, Stockholm, Sweden.,Affinity Proteomics; SciLifeLab; School of Biotechnology; KTH-Royal Institute of Technology; Stockholm Sweden(Swepub:kth)u1ws88sk (author)
  • van Hage, M. (author)
  • Karolinska InstitutetImmunology and Allergy Unit; Department of Medicine Solna; Karolinska Institutet; and Karolinska University Hospital;Center for Inflammatory Diseases; Karolinska Institutet; Stockholm Sweden (creator_code:org_t)

Related titles

  • In:Allergy. European Journal of Allergy and Clinical Immunology: Blackwell Publishing71:9, s. 1357-13610105-45381398-9995

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