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Biocatalysis on the...
Biocatalysis on the surface of Escherichia coli : melanin pigmentation of the cell exterior
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- Gustavsson, Martin, 1984- (author)
- KTH,Industriell bioteknologi,Gen Larsson
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- Hörnström, David (author)
- KTH,Industriell bioteknologi,Gen Larsson
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- Lundh, Susanna (author)
- KTH,Industriell bioteknologi,Gen Larsson
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- Belotserkovsky, Jaroslav (author)
- KTH,Industriell bioteknologi
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- Larsson, Gen (author)
- KTH,Industriell bioteknologi
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(creator_code:org_t)
- 2016-10-26
- 2016
- English.
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In: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 6
- Related links:
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https://doi.org/10.1...
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https://kth.diva-por... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Today, it is considered state-of-the-art to engineer living organisms for various biotechnology applications. Even though this has led to numerous scientific breakthroughs, the enclosed interior of bacterial cells still restricts interactions with enzymes, pathways and products due to the mass-transfer barrier formed by the cell envelope. To promote accessibility, we propose engineering of biocatalytic reactions and subsequent product deposition directly on the bacterial surface. As a proof-of-concept, we used the AIDA autotransporter vehicle for Escherichia coli surface expression of tyrosinase and fully oxidized externally added tyrosine to the biopolymer melanin. This resulted in a color change and creation of a black cell exterior. The capture of ninety percent of a pharmaceutical wastewater pollutant followed by regeneration of the cell bound melanin matrix through a simple pH change, shows the superior function and facilitated processing provided by the surface methodology. The broad adsorption spectrum of melanin could also allow removal of other micropollutants.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
Keyword
- Expression
- Autotransporter
- Purification
- Tyrosinase
- Proteins
- Enzymes
- Design
- Gene
- Bioteknologi
- Biotechnology
Publication and Content Type
- ref (subject category)
- art (subject category)
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