Acoustic micro-vortexing of fluids, beads and cells in disposible microfluidic chips

Ramachandraiah, Harisha (author): KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab

Russom, Aman (author): KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab

(creator_code:org_t)

Chemical and Biological Microsystems Society, 2015
2015
English.
In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 1005-1007

Abstract Subject headings

In this paper we demonstrate a multi-functional platform using ultrasound for vortexing of 20-μl volumes of different samples in polymer-based disposable chips. The method enables different vortexing functions such as mixing laminar flows, resuspension of a micro-pellet of magnetic beads as well as cell lysis for DNA extraction. The device consists of an inexpensive low-frequency, high power, horn-shaped langevin transducer which is typically used for cell disruption in larger volumes. By controlling the operating time of this device (from fractions of a second up to a minute) different functions can be achieved. In addition, to avoid the high-power-induced heating, a simple cooling system is used as a chip holder consisting of a PC fan-cooled aluminum heat sink. To demonstrate a sample preparation application, we perform on-chip cell lysis and DNA extraction.

By the author/editor: Iranmanesh, Ida; Ohlin, Mathias; Ramachandraiah, ...; Russom, Aman; Wiklund, Martin

About the subject

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