Ultrahigh sensitivity made simple: nanoplasmonic label-free biosensing with an extremely low limit-of-detection for bacterial and cancer diagnostics.

Ultrahigh sensitivity made simple : nanoplasmonic label-free biosensing with an extremely low limit-of-detection for bacterial and cancer diagnostics.

Chen, Si, 1985 (author): Chalmers tekniska högskola,Chalmers University of Technology

Svedendahl, M (author): Chalmers tekniska högskola,Chalmers University of Technology

Käll, Mikael, 1963 (author): Chalmers tekniska högskola,Chalmers University of Technology

Gunnarsson, Linda K, 1971 (author): Chalmers tekniska högskola,Chalmers University of Technology

Dmitriev, Alexander, 1975 (author): Chalmers tekniska högskola,Chalmers University of Technology

(creator_code:org_t)

2009-10-02
2009
English.
In: Nanotechnology. - : Institute of Physics (IOP). - 0957-4484 .- 1361-6528. ; 20:43

Related links:: http://dx.doi.org/10...; show more...; https://urn.kb.se/re...; https://doi.org/10.1...; https://research.cha...; show less...

Abstract Subject headings

We present a simple and robust scheme for biosensing with an ultralow limit-of-detection down to several pg cm(-2) (or several tens of attomoles cm(-2)) based on optical label-free biodetection with localized surface plasmon resonances. The scheme utilizes cost-effective optical components and comprises a white light source, a properly functionalized sensor surface enclosed in a simple fluidics chip, and a spectral analyzer. The sensor surface is produced by a bottom-up nanofabrication technique with hole mask colloidal lithography. Despite its simplicity, the method is able to reliably detect protein-protein binding events at low picomolar and femtomolar concentrations, which is exemplified by the label-free detection of the extracellular adherence protein (EAP) found on the outer surface of the bacterium Staphylococcus aureus and of prostate-specific antigen (PSA), which is believed to be a prostate cancer marker. These experiments pave the way towards an ultra-sensitive yet compact biodetection platform for point-of-care diagnostics applications.

TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)

By the author/editor: Chen, Si, 1985; Svedendahl, M; Käll, Mikael, 19 ...; Gunnarsson, Lind ...; Dmitriev, Alexan ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Physical Science ...; and Atom and Molecul ...

NATURAL SCIENCES: NATURAL SCIENCES; and Physical Science ...; and Other Physics To ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

By the university: Royal Institute of Technology; Chalmers University of Technology

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