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Partial protection ...
Partial protection to respiratory syncytial virus (RSV) elicited in mice by intranasal immunization using live staphylococci with surface-displayed RSV-peptides
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Cano, F. (author)
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Plotnicky-Gilquin, H. (author)
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Nguyen, T. N. (author)
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Liljeqvist, S. (author)
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- Samuelson, Patrik (author)
- KTH,Bioteknologi
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Bonnefoy, J. Y. (author)
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- Ståhl, Stefan (author)
- KTH,Bioteknologi
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Robert, A. (author)
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(creator_code:org_t)
- 2000
- 2000
- English.
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In: Vaccine. - 0264-410X .- 1873-2518. ; 18:24, s. 2743-2752
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- A live bacterial vaccine-delivery system based on the food-grade bacterium Staphylococcus carnosus was used for delivery of peptides from the G glycoprotein of human respiratory syncytial virus, subtype A (RSV-A). Three peptides, corresponding to the G protein amino acids, 144-159 (denoted G5), 190-203 (G9) and 171-188 (G4 S), the latter with four cysteine residues substituted for serines, were expressed by recombinant means as surface-exposed on three different bacteria, and their surface accessibility on the bacteria was verified by fluorescence-activated cell sorting (FACS). Intranasal immunization of mice with the live recombinant staphylococci elicited significant anti-peptide as well as anti-virus serum IgG responses of balanced IgG1/IgG2a isotype profiles, and upon viral challenge with 10(5) tissue culture infectious doses(50) (TCID50), lung protection was demonstrated for approximately half of the mice in the G9 and G4 S immunization groups. To our knowledge, this is the first study in which protective immunity to a viral pathogen has been evoked using food-grade bacteria as vaccine-delivery vehicles.
Keyword
- live bacterial vaccine delivery
- respiratory syncytial virus
- Staphylococcus carnosus
- recombinant lactococcus-lactis
- streptococcal protein-g
- albumin-binding region
- g fusion protein
- g-glycoprotein
- cell-wall
- klebsiella-pneumoniae
- antibody-responses
- oral vaccination
- balb/c mice
Publication and Content Type
- ref (subject category)
- art (subject category)
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