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Spontaneous Vesicul...
Spontaneous Vesiculation and pH-Induced Disassembly of a Lysosomotropic Detergent : Impacts on Lysosomotropism and Lysosomal Delivery
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- Villamil Giraldo, Ana Maria (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
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- Fyrner, Timmy (author)
- Linköpings universitet,Tekniska fakulteten,Kemi
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- Wennmalm, Stefan (author)
- KTH,Tillämpad fysik,Royal Institute Technology, Sweden
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- Parikh, Atul N. (author)
- University of Calif Davis, CA 95616 USA; University of Calif Davis, CA 95616 USA
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- Öllinger, Karin (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Region Östergötland, Klinisk patologi och klinisk genetik
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- Ederth, Thomas (author)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
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(creator_code:org_t)
- 2016-12-12
- 2016
- English.
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In: LANGMUIR. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 32:50, s. 13566-13575
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Lysosomotropic detergents (LDs) selectively rupture lysosomal membranes through mechanisms that have yet to be characterized. A consensus view, currently, holds that LDs, which are weakly basic, diffuse across cellular membranes as monomers in an uncharged state, and via protonation in the acidic lysosomal compartment, they become trapped, accumulate, and subsequently solubilize the membrane and induce lysosomal membrane permeabilization. Here we demonstrate that the lysosomotropic detergent O-methyl-serine dodecylamide hydrochloride (MSDH) spontaneously assembles into vesicles at, and above, cytosolic pH, and that the vesicles disassemble as the pH reaches 6.4 or lower. The aggregation commences at concentrations below the range of those used in cell studies. Assembly and disassembly of the vesicles was studied via dynamic light scattering, zeta potential measurements, cryo-TEM, and fluorescence correlation spectroscopy and was found to be reversible via control of the pH. Aggregation of MSDH into closed vesicles under cytosolic conditions is at variance with the commonly held view of LD behavior, and we propose that endocytotic pathways should be considered as possible routes of LD entry into lysosomes. We further demonstrate that MSDH vesicles can be loaded with fluorophores via a solution transition from low to high pH, for subsequent release when the pH is lowered again. The ability to encapsulate molecular cargo into MSDH vesicles together with its ability to disaggregate at low pH and to permeabilize the lysosomal membrane presents an intriguing possibility to use MSDH as a delivery system.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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