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  • Enfors, Sven-OlofKTH,Bioteknologi (author)

Physiological responses to mixing in large scale bioreactors

  • Article/chapterEnglish2001

Publisher, publication year, extent ...

  • 2001
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:kth-20371
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-20371URI
  • https://doi.org/10.1016/S0168-1656(00)00365-5DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • QC 20100525
  • Escherichia coli fed-batch cultivations at 22 m(3) scale were compared to corresponding laboratory scale processes and cultivations using a scale-down reactor furnished with a high-glucose concentration zone to mimic the conditions in a feed zone of the large bioreactor. Formate accumulated in the large reactor, indicating the existence of oxygen limitation zones. It is suggested that the reduced biomass yield at large scale partly is due to repeated production/reassimilation of acetate from overflow metabolism and mixed acid fermentation products due to local moving zones with oxygen limitation. The conditions that generated mixed-acid fermentation in the scale-down reactor also induced a number of stress responses, monitored by analysis of mRNA of selected stress induced genes. The stress responses were relaxed when the cells returned to the substrate limited and oxygen sufficient compartment of the reactor. Corresponding analysis in the large reactor showed that the concentration of mRNA of four stress induced genes was lowest at the sampling port most distant from the feed zone. It is assumed that repeated induction/relaxation of stress responses in a large bioreactor may contribute to altered physiological properties of the cells grown in large-scale bioreactor. Flow cytometric analysis revealed reduced damage with respect to cytoplasmic membrane potential and integrity in cells grown in the dynamic environments of the large scale reactor and the scale-down reactor.

Subject headings and genre

  • scale-up
  • Escherichia coli
  • mixed-acid fermentation
  • stress responses
  • concentration gradients
  • CFD
  • fed-batch cultures
  • escherichia-coli
  • saccharomyces-cerevisiae
  • overflow metabolism
  • growth

Added entries (persons, corporate bodies, meetings, titles ...)

  • Jahic, M. (author)
  • Rozkov, A. (author)
  • Xu, B. (author)
  • Hecker, M. (author)
  • Jurgen, B. (author)
  • Kruger, E. (author)
  • Schweder, T. (author)
  • Hamer, G. (author)
  • O'Beirne, D. (author)
  • Noisommit-Rizzi, N. (author)
  • Reuss, M. (author)
  • Boone, L. (author)
  • Hewitt, C. (author)
  • McFarlane, C. (author)
  • Nienow, A. (author)
  • Kovacs, T. (author)
  • Tragardh, C. (author)
  • Fuchs, Laszlo (author)
  • Revstedt, J. (author)
  • Friberg, P. C. (author)
  • Hjertager, B. (author)
  • Blomsten, G. (author)
  • Skogman, H. (author)
  • Hjort, S. (author)
  • Hoeks, F. (author)
  • Lin, H. Y. (author)
  • Neubauer, P. (author)
  • van der Lans, R. (author)
  • Luyben, K. (author)
  • Vrabel, P. (author)
  • Manelius, A. (author)
  • KTHBioteknologi (creator_code:org_t)

Related titles

  • In:Journal of Biotechnology85:2, s. 175-1850168-16561873-4863

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