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Determination of th...
Determination of the maximum specific uptake capacities for glucose and oxygen in glucose-limited fed-batch cultivations of Escherichia coli
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Lin, H. Y. (author)
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Mathiszik, B. (author)
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Xu, B. (author)
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- Enfors, Sven-Olof (author)
- KTH,Bioteknologi
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Neubauer, P. (author)
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(creator_code:org_t)
- 2001
- 2001
- English.
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In: Biotechnology and Bioengineering. - : Wiley. - 0006-3592 .- 1097-0290. ; 73:5, s. 347-357
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- A simple pulse-based method for the determination of the maximum uptake capacities for glucose a nd oxygen in glucose limited cultivations of E. coli is presented. The method does not depend on the time-consuming analysis of glucose or acetate, and therefore can be used to control the feed rate in glucose limited cultivations, such as fed-batch processes. The application of this method in fed-batch processes of E. coli showed that the uptake capacity for neither glucose nor oxygen is a constant parameter, as often is assumed in fed-batch models. The glucose uptake capacity decreased significantly when the specific growth rate decreased below 0.15 h(-1) and fell to about 0.6 mmol g(-1) h(-1) (mmol per g cell dry weight and hour) at the end of fed-batch fermentations, where mu was approximately 0.02 h(-1). The oxygen uptake capacity started to decrease somewhat earlier when mu declined below 0.25 h(-1) and was 5 mmol g(-1) h(-1) at the end of the fermentations. The behavior of both uptake systems is integrated in a dynamic model which allows a better fitting of experimental values for glucose in fed-batch processes in comparison to generally used unstructured kinetic models.
Keyword
- fed-batch-fermentation
- kinetic model
- acetate
- oxygen uptake
- dissolved oxygen tension
- high-cell-density
- recombinant protein-production
- phenylalanine production
- human interleukin-2
- fermenter cultures
- acetate formation
- feeding strategy
- growth
- expression
- metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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