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- Persson, HelenaKTH,Proteinteknologi,Science for Life Laboratory, SciLifeLab,Drug Discovery and Development Platform (author)
Antibody validation by immunoprecipitation followed by mass spectrometry analysis
- Article/chapterEnglish2017
Publisher, publication year, extent ...
- 2017-03-03
- New York, NY :Humana Press Inc.2017
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:kth-207430
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-207430URI
- https://doi.org/10.1007/978-1-4939-6857-2_10DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:228255880URI
Supplementary language notes
- Language:English
- Summary in:English
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- QC 20170523
- We describe a mass spectrometry-based approach for validation of antibody specificity. This method allows validation of antibodies or antibody fragments, against their endogenous targets. It can assess if the antibody is able to bind to its native antigen in cell lysates among thousands of other proteins, DNA, RNA, and other cellular components. In addition, it identifies other proteins the antibody is able to immunoprecipitate allowing for the assessment of antibody specificity and selectivity. This method is easily scalable, adaptable to different cell lines and conditions and has been shown to be reproducible between multiple laboratories.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
- Antibody fragments
- Antibody validation
- Immunoprecipitation
- Mass spectrometry
Added entries (persons, corporate bodies, meetings, titles ...)
- Preger, C.Karolinska Institutet (author)
- Marcon, E. (author)
- Lengqvist, J.Karolinska Institutet (author)
- Gräslund, S.Karolinska Institutet (author)
- KTHProteinteknologi (creator_code:org_t)
Related titles
- In:Methods in Molecular BiologyNew York, NY : Humana Press Inc.1575, s. 175-187
- In:Methods in molecular biology (Clifton, N.J.)New York, NY : Humana Press Inc.1575, s. 175-1871940-6029
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