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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02736naa a2200445 4500 | |
| 001 | oai:DiVA.org:kth-207430 | |
| 003 | SwePub | |
| 008 | 170523s2017 | |||||||||||000 ||eng| | |
| 009 | oai:prod.swepub.kib.ki.se:228255880 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2074302 URI |
| 024 | 7 | a https://doi.org/10.1007/978-1-4939-6857-2_102 DOI |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:2282558802 URI |
| 040 | a (SwePub)kthd (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a kon2 swepub-publicationtype |
| 100 | 1 | a Persson, Helenau KTH,Proteinteknologi,Science for Life Laboratory, SciLifeLab,Drug Discovery and Development Platform4 aut0 (Swepub:kth)u1l700sh |
| 245 | 1 0 | a Antibody validation by immunoprecipitation followed by mass spectrometry analysis |
| 264 | c 2017-03-03 | |
| 264 | 1 | a New York, NY :b Humana Press Inc.c 2017 |
| 338 | a print2 rdacarrier | |
| 500 | a QC 20170523 | |
| 520 | a We describe a mass spectrometry-based approach for validation of antibody specificity. This method allows validation of antibodies or antibody fragments, against their endogenous targets. It can assess if the antibody is able to bind to its native antigen in cell lysates among thousands of other proteins, DNA, RNA, and other cellular components. In addition, it identifies other proteins the antibody is able to immunoprecipitate allowing for the assessment of antibody specificity and selectivity. This method is easily scalable, adaptable to different cell lines and conditions and has been shown to be reproducible between multiple laboratories. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng |
| 653 | a Antibody fragments | |
| 653 | a Antibody validation | |
| 653 | a Immunoprecipitation | |
| 653 | a Mass spectrometry | |
| 700 | 1 | a Preger, C.u Karolinska Institutet4 aut |
| 700 | 1 | a Marcon, E.4 aut |
| 700 | 1 | a Lengqvist, J.u Karolinska Institutet4 aut |
| 700 | 1 | a Gräslund, S.u Karolinska Institutet4 aut |
| 710 | 2 | a KTHb Proteinteknologi4 org |
| 773 | 0 | t Methods in Molecular Biologyd New York, NY : Humana Press Inc.g 1575, s. 175-187q <175-187 |
| 773 | 0 | t Methods in molecular biology (Clifton, N.J.)d New York, NY : Humana Press Inc.g 1575, s. 175-187q 1575<175-187x 1940-6029 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-207430 |
| 856 | 4 8 | u https://doi.org/10.1007/978-1-4939-6857-2_10 |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:228255880 |
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