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Labeling of human C...
Labeling of human C-peptide by conjugation with N-succinimidyl-4- F-18 fluorobenzoate
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Fredriksson, A. (author)
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- Johnstrom, P. (author)
- Karolinska Institutet
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- Stone-Elander, S. (author)
- Karolinska Institutet
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Jonasson, P. (author)
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- Nygren, Per-Åke (author)
- KTH,Bioteknologi
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Ekberg, K. (author)
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Johansson, B. L. (author)
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- Wahren, J. (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2001-05-25
- 2001
- English.
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In: Journal of labelled compounds & radiopharmaceuticals. - : Wiley. - 0362-4803 .- 1099-1344. ; 44:7, s. 509-519
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Subject headings
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- We have labeled proinsulin connecting peptide (C-peptide) with fluorine-18 (t(1/2) = 109.7min) in order to perform in vivo biodistribution and pharmacokinetic studies with position emission tomography (PET). This study reports the optimization of the conjugation labeling in the N-terminal with N-succinimidyl-4-[F-18]fluorobenzoate ([F-18]SFB). In preparative runs N-4-[F-18]fluorobenzoyl-C-peptide ([F-18]FB-C-peptide) was produced in 8-12% decay-corrected yields, counted from resolubilized [F-18]F-, in less than 5h. The specific radioactivity of [F-18]FB-C-peptide, determined using ELISA for one of the preparations, was around 70 GBq/mu mol at end of synthesis.
Keyword
- fluorine-18
- protein labeling
- connecting peptide
- diabetes
- PET
- n-succinimidyl 4-<f-18>fluorobenzoate
- f-18
- proteins
- exchange
Publication and Content Type
- ref (subject category)
- art (subject category)
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