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Experimental strate...
Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
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- Daurer, Benedikt J. (author)
- Uppsala universitet,Molekylär biofysik
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- Okamoto, Kenta (author)
- Uppsala universitet,Molekylär biofysik
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- Bielecki, Johan (author)
- Uppsala universitet,Molekylär biofysik
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- Maia, Filipe R. N. C. (author)
- Uppsala universitet,Molekylär biofysik,Science for Life Laboratory, SciLifeLab
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- Mühlig, Kerstin (author)
- Uppsala universitet,Molekylär biofysik
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- Seibert, M. Marvin (author)
- Uppsala universitet,Molekylär biofysik
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- Hantke, Max F. (author)
- Uppsala universitet,Molekylär biofysik
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Nettelblad, Carl (author)
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Benner, W. Henry (author)
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- Svenda, Martin (author)
- Uppsala universitet,Molekylär biofysik
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- Tîmneanu, Nicuşor (author)
- Uppsala universitet,Molekyl- och kondenserade materiens fysik
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Ekeberg, Tomas (author)
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Loh, N. Duane (author)
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- Pietrini, Alberto (author)
- Uppsala universitet,Molekylär biofysik
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- Zani, Alessandro (author)
- Uppsala universitet,Molekylär biofysik
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- Rath, Asawari D. (author)
- Uppsala universitet,Molekylär biofysik
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- Westphal, Daniel (author)
- Uppsala universitet,Molekylär biofysik
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Kirian, Richard A. (author)
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Awel, Salah (author)
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Wiedorn, Max O. (author)
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- van der Schot, Gijs (author)
- Uppsala universitet,Molekylär biofysik
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- Carlsson, Gunilla H. (author)
- Uppsala universitet,Molekylär biofysik
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- Hasse, Dirk (author)
- Uppsala universitet,Molekylär biofysik
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- Sellberg, Jonas A. (author)
- Uppsala universitet,KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova,Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden,Molekylär biofysik
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Barty, Anton (author)
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- Andreasson, Jakob (author)
- Uppsala universitet,Molekylär biofysik
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Boutet, Sebastian (author)
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Williams, Garth (author)
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Koglin, Jason (author)
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- Andersson, Inger (author)
- Uppsala universitet,Molekylär biofysik
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- Hajdu, Janos (author)
- Uppsala universitet,Molekylär biofysik
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- Larsson, Daniel S. D. (author)
- Uppsala universitet,Molekylär biofysik
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(creator_code:org_t)
- INT UNION CRYSTALLOGRAPHY, 2017
- 2017
- English.
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In: IUCrJ. - : INT UNION CRYSTALLOGRAPHY. - 2052-2525. ; 4, s. 251-262
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
Close
- This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of similar to 40 nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from similar to 35 to similar to 300 nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 * 10(12) photons per mu m(2) per pulse. The full-width of the focus at half-maximum was estimated to be 500 nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.
Subject headings
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Keyword
- X-ray diffraction
- free-electron laser
- flash X-ray imaging
- diffraction before destruction
- virus
- Omono River virus
- OmRV
Publication and Content Type
- ref (subject category)
- art (subject category)
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IUCrJ
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To the university's database
- By the author/editor
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Daurer, Benedikt ...
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Okamoto, Kenta
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Bielecki, Johan
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Maia, Filipe R. ...
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Mühlig, Kerstin
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Seibert, M. Marv ...
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show more...
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Hantke, Max F.
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Nettelblad, Carl
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Benner, W. Henry
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Svenda, Martin
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Tîmneanu, Nicuşo ...
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Ekeberg, Tomas
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Loh, N. Duane
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Pietrini, Albert ...
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Zani, Alessandro
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Rath, Asawari D.
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Westphal, Daniel
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Kirian, Richard ...
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Awel, Salah
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Wiedorn, Max O.
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van der Schot, G ...
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Carlsson, Gunill ...
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Hasse, Dirk
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Sellberg, Jonas ...
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Barty, Anton
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Andreasson, Jako ...
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Boutet, Sebastia ...
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Williams, Garth
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Koglin, Jason
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Andersson, Inger
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Hajdu, Janos
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Larsson, Daniel ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
- Articles in the publication
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IUCrJ
- By the university
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Royal Institute of Technology
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Uppsala University