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Experimental strate...
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Daurer, Benedikt J.Uppsala universitet,Molekylär biofysik
(author)
Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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INT UNION CRYSTALLOGRAPHY,2017
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-207895
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-207895URI
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https://doi.org/10.1107/S2052252517003591DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-323439URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Correction in DOI: 10.1107/S2052252519004317 WOS: 000466861200017QC 20170530
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This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of similar to 40 nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from similar to 35 to similar to 300 nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 * 10(12) photons per mu m(2) per pulse. The full-width of the focus at half-maximum was estimated to be 500 nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.
Subject headings and genre
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Okamoto, KentaUppsala universitet,Molekylär biofysik(Swepub:uu)kenok266
(author)
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Bielecki, JohanUppsala universitet,Molekylär biofysik(Swepub:uu)johbi267
(author)
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Maia, Filipe R. N. C.Uppsala universitet,Molekylär biofysik,Science for Life Laboratory, SciLifeLab(Swepub:uu)carne864
(author)
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Mühlig, KerstinUppsala universitet,Molekylär biofysik(Swepub:uu)kermu539
(author)
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Seibert, M. MarvinUppsala universitet,Molekylär biofysik(Swepub:uu)masei255
(author)
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Hantke, Max F.Uppsala universitet,Molekylär biofysik(Swepub:uu)maxha207
(author)
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Nettelblad, Carl
(author)
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Benner, W. Henry
(author)
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Svenda, MartinUppsala universitet,Molekylär biofysik(Swepub:uu)msv31659
(author)
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Tîmneanu, NicuşorUppsala universitet,Molekyl- och kondenserade materiens fysik(Swepub:uu)nti19847
(author)
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Ekeberg, Tomas
(author)
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Loh, N. Duane
(author)
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Pietrini, AlbertoUppsala universitet,Molekylär biofysik(Swepub:uu)albpi258
(author)
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Zani, AlessandroUppsala universitet,Molekylär biofysik(Swepub:uu)aleza299
(author)
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Rath, Asawari D.Uppsala universitet,Molekylär biofysik
(author)
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Westphal, DanielUppsala universitet,Molekylär biofysik(Swepub:uu)danwe345
(author)
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Kirian, Richard A.
(author)
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Awel, Salah
(author)
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Wiedorn, Max O.
(author)
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van der Schot, GijsUppsala universitet,Molekylär biofysik(Swepub:uu)gijva150
(author)
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Carlsson, Gunilla H.Uppsala universitet,Molekylär biofysik(Swepub:uu)gca29842
(author)
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Hasse, DirkUppsala universitet,Molekylär biofysik(Swepub:uu)dirha931
(author)
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Sellberg, Jonas A.Uppsala universitet,KTH,Biomedicinsk fysik och röntgenfysik,Albanova VinnExcellence Center for Protein Technology, ProNova,Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden,Molekylär biofysik(Swepub:kth)u1ynvdbz
(author)
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Barty, Anton
(author)
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Andreasson, JakobUppsala universitet,Molekylär biofysik(Swepub:uu)jakan998
(author)
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Boutet, Sebastian
(author)
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Williams, Garth
(author)
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Koglin, Jason
(author)
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Andersson, IngerUppsala universitet,Molekylär biofysik(Swepub:uu)inand172
(author)
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Hajdu, JanosUppsala universitet,Molekylär biofysik(Swepub:uu)janohajd
(author)
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Larsson, Daniel S. D.Uppsala universitet,Molekylär biofysik(Swepub:uu)dalar193
(author)
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Uppsala universitetMolekylär biofysik
(creator_code:org_t)
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In:IUCrJ: INT UNION CRYSTALLOGRAPHY4, s. 251-2622052-2525
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Daurer, Benedikt ...
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Okamoto, Kenta
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Bielecki, Johan
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Maia, Filipe R. ...
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Mühlig, Kerstin
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Seibert, M. Marv ...
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Hantke, Max F.
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Nettelblad, Carl
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Benner, W. Henry
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Svenda, Martin
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Tîmneanu, Nicuşo ...
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Ekeberg, Tomas
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Loh, N. Duane
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Pietrini, Albert ...
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Zani, Alessandro
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Rath, Asawari D.
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Westphal, Daniel
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Kirian, Richard ...
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Awel, Salah
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Wiedorn, Max O.
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van der Schot, G ...
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Carlsson, Gunill ...
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Hasse, Dirk
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Sellberg, Jonas ...
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Barty, Anton
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Andreasson, Jako ...
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Boutet, Sebastia ...
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Williams, Garth
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Koglin, Jason
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Andersson, Inger
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Hajdu, Janos
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Larsson, Daniel ...
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