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Multi-level charact...
Multi-level characterization of balanced inhibitory-excitatory cortical neuron network derived from human pluripotent stem cells
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Nadadhur, A. G. (author)
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Melero, J. E. (author)
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Meijer, M. (author)
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Schut, D. (author)
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Jacobs, G. (author)
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Wan Li, K. (author)
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- Hjorth, Johannes (author)
- KTH,Beräkningsvetenskap och beräkningsteknik (CST),Science for Life Laboratory, SciLifeLab,Vrije Universiteit, Netherlands
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Meredith, R. M. (author)
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Toonen, R. F. (author)
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Van Kesteren, R. E. (author)
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Smit, A. B. (author)
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Verhage, M. (author)
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Heine, V. M. (author)
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(creator_code:org_t)
- 2017-06-06
- 2017
- English.
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In: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 12:6
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory- inhibitory activities, which are suitable for single cell analysis. We generated low-density networks of hPSC-derived GABAergic and glutamatergic cortical neurons. We used two different co-culture models with astrocytes. We show that these cultures have balanced excitatory-inhibitory synaptic identities using confocal microscopy, electrophysiological recordings, calcium imaging and mRNA analysis. These simple and robust protocols offer the opportunity for single-cell to multi-level analysis of patient hiPSC-derived cortical excitatory- inhibitory networks; thereby creating advanced tools to study disease mechanisms underlying neurodevelopmental disorders.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Ganglionic Eminence
- Astrocyte Coculture
- Differentiation
- Interneurons
- Brain
- Gaba
- Specification
- Maturation
- Glutamate
- Disease
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Nadadhur, A. G.
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Melero, J. E.
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Meijer, M.
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Schut, D.
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Jacobs, G.
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Wan Li, K.
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show more...
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Hjorth, Johannes
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Meredith, R. M.
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Toonen, R. F.
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Van Kesteren, R. ...
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Smit, A. B.
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Verhage, M.
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Heine, V. M.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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PLOS ONE
- By the university
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Royal Institute of Technology