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Streamlined Prepara...
Streamlined Preparation of Immobilized Candida antarctica Lipase B
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- Cassimjee, Karim Engelmark (author)
- EnginZyme AB
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- Hendil-Forssell, Peter (author)
- EnginZyme AB
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- Volkov, Alexey (author)
- EnginZyme AB
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- Krog, Anne (author)
- Vectron Biosolutions AS
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- Malmo, Jostein (author)
- Vectron Biosolutions AS
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- Aune, Trond Erik V. (author)
- Vectron Biosolutions AS
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- Knecht, Wolfgang (author)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science
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- Miskelly, Iain R. (author)
- Almac Group Limited,Arran Chemical Company Limited
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- Moody, Thomas S. (author)
- Arran Chemical Company Limited
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- Humble, Maria Svedendahl (author)
- KTH Royal Institute of Technology,KTH,Industriell bioteknologi,Pharem Biotech AB
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(creator_code:org_t)
- 2017-12-06
- 2017
- English.
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In: ACS Omega. - : American Chemical Society (ACS). - 2470-1343. ; 2:12, s. 8674-8677
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http://dx.doi.org/10...
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Abstract
Subject headings
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- Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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ACS Omega
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- By the author/editor
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Cassimjee, Karim ...
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Hendil-Forssell, ...
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Volkov, Alexey
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Krog, Anne
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Malmo, Jostein
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Aune, Trond Erik ...
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Knecht, Wolfgang
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Miskelly, Iain R ...
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Moody, Thomas S.
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Humble, Maria Sv ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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ACS Omega
- By the university
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Royal Institute of Technology
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Lund University