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Recruitment of rena...
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Kruse, M. S.
(författare)
Recruitment of renal dopamine 1 receptors requires an intact microtubulin network
- Artikel/kapitelEngelska2003
Förlag, utgivningsår, omfång ...
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Springer Science and Business Media LLC,2003
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:kth-22354
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-22354URI
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https://doi.org/10.1007/s00424-002-0899-5DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1929406URI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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QC 20100525
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Renal dopamine1 receptor (D1R) can be recruited from intracellular compartments to the plasma membrane by D1R agonists and endogenous dopamine. This study examines the role of the cytoskeleton for renal D1R recruitment. The studies were performed in LLCPK-1 cells that have the capacity to form dopamine from L-dopa. In approximately 50% of the cells treated with L-dopa the D1R was found to be translocated from intracellular compartments towards the plasma membrane. Disruption of the microtubulin network by noco-dazole significantly prevented translocation. In contrast, depolymerization of actin had no effect. In control cells D1R colocalized with NBD-C-6-ceramide, a trans-Golgi fluorescent marker. This colocalization was disrupted in L-dopa-treated cells. Tetanus toxin, an inhibitor of exocytosis, prevented L-dopa-induced receptor recruitment. L-Dopa treatment resulted in activation of protein kinase C (PKC). To test the functional effect of D1R recruitment, the capacity of D1R agonists to activate PKC was studied. Activation of D1R significantly translocated PKC-alpha from intracellular compartments to the plasma membrane. Disruption of microtubules abolished D1R-mediated - but not phorbol-ester-mediated - translocation of PKC. We conclude that renal D1R recruitment requires an intact microtubulin network and occurs via Golgi-derived vesicles. These newly recruited receptors couple to the PKC signaling pathway.
Ämnesord och genrebeteckningar
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G-protein-coupled receptors
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kidney
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cytoskeleton
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protein kinase C
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vesicles
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protein-coupled receptors
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trans-golgi network
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epithelial-cells
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plasma-membrane
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d1 receptors
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mechanism
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Adachi, S.
(författare)
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Scott, L.Karolinska Institutet
(författare)
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Holtback, U.
(författare)
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Greengard, P.
(författare)
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Aperia, A.Karolinska Institutet
(författare)
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Brismar, HjalmarKarolinska Institutet(Swepub:kth)u1232ew5
(författare)
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Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Pflügers Archiv: Springer Science and Business Media LLC445:5, s. 534-5390031-67681432-2013
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