A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitidis

• Paper-based biosensors offer a promising technology to be used at the point of care, enabled by good performance, convenience and low-cost. In this article, we describe a colorimetric vertical-flow DNA microarray (DNA-VFM) that takes advantage of the screening capability of DNA microarrays in a paper format together with isothermal amplification by means of Recombinase Polymerase Amplification (RPA). Different assay parameters such as hybridization buffer, flow rate, printing buffer and capture probe concentration were optimized. A limit of detection (LOD) of 4.4 nM was achieved as determined by tabletop scanning. The DNA-VFM was applied as a proof of concept for detection of Neisseria meningitidis, a primary cause of bacterial meningitis. The LOD was determined to be between 38 and 2.1Å~106 copies/VFM assay, depending on the choice of DNA capture probes. The presented approach provides multiplex capabilities of DNA microarrays in a paper-based format for future point-of-care applications.

Subject headings

TEKNIK OCH TEKNOLOGIER  -- Miljöbioteknik -- Diagnostisk bioteknologi (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Environmental Biotechnology -- Diagnostic Biotechnology (hsv//eng)

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Keyword

Microarray

Paper-based biosensor

Point-of-care

Gold nanoparticles

Neisseria meningitidis

Publication and Content Type

ref (subject category)

art (subject category)